Detailed information of TA system TA077382

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5936625..5937211
• Replicon: chromosome
• Accession: NZ_CP021775
• Organism: Pseudomonas aeruginosa strain Pa58

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: CDL16_RS28810
• Protein ID: WP_003120987.1
• Coordinates: 5936912..5937211 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: CDL16_RS28805
• Protein ID: WP_003448662.1
• Coordinates: 5936625..5936915 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDL16_RS28790	5932206..5934095	+	1890	WP_023093670.1	hypothetical protein	-
CDL16_RS28795	5934092..5936068	+	1977	WP_023093671.1	DEAD/DEAH box helicase	-
CDL16_RS28800	5936207..5936542	+	336	WP_022579623.1	hypothetical protein	-
CDL16_RS28805	5936625..5936915	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
CDL16_RS28810	5936912..5937211	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDL16_RS28815	5937413..5938537	+	1125	WP_023093672.1	TcpQ domain-containing protein	-
CDL16_RS28820	5938537..5940246	+	1710	WP_023093673.1	PilN family type IVB pilus formation outer membrane protein	-
CDL16_RS28825	5940250..5941575	+	1326	WP_022579625.1	type 4b pilus protein PilO2	-
CDL16_RS28830	5941565..5942098	+	534	WP_003149521.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5906126..5994299	88173

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T77382 WP_003120987.1 NZ_CP021775:c5937211-5936912 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T77382 NZ_CP021775:c5937211-5936912 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT77382 WP_003448662.1 NZ_CP021775:c5936915-5936625 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT77382 NZ_CP021775:c5936915-5936625 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
CTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCTCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCGACATTGAAAACCATGCTGGCA
GTAATGCGAGCTTTGGGCGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73