Detailed information of TA system TA077374

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 157620..158125
• Replicon: chromosome
• Accession: NZ_CP021775
• Organism: Pseudomonas aeruginosa strain Pa58

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: CDL16_RS00730
• Protein ID: WP_003083773.1
• Coordinates: 157620..157901 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: CDL16_RS00735
• Protein ID: WP_003083775.1
• Coordinates: 157898..158125 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDL16_RS00705	152871..154220	+	1350	WP_003119513.1	C4-dicarboxylate transporter DctA	-
CDL16_RS00710	154269..154955	+	687	WP_003083762.1	FadR family transcriptional regulator	-
CDL16_RS00715	155056..155790	+	735	WP_003128333.1	GntR family transcriptional regulator	-
CDL16_RS00720	155994..156380	+	387	WP_003083767.1	aegerolysin family protein	-
CDL16_RS00725	156412..157320	-	909	WP_003083769.1	LysR family transcriptional regulator	-
CDL16_RS00730	157620..157901	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
CDL16_RS00735	157898..158125	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
CDL16_RS00740	158301..158921	-	621	WP_003101226.1	hypothetical protein	-
CDL16_RS00745	159022..159522	+	501	WP_003128336.1	LEA type 2 family protein	-
CDL16_RS00750	159595..159936	+	342	WP_003101229.1	alkylphosphonate utilization protein	-
CDL16_RS00755	160018..161445	-	1428	WP_003083784.1	GABA permease	-
CDL16_RS00760	161614..163107	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T77374 WP_003083773.1 NZ_CP021775:c157901-157620 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T77374 NZ_CP021775:c157901-157620 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT77374 WP_003083775.1 NZ_CP021775:c158125-157898 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT77374 NZ_CP021775:c158125-157898 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9