Detailed information of TA system TA077364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 1443863..1444971
• Replicon: chromosome
• Accession: NZ_CP021773
• Organism: Streptococcus agalactiae strain B105

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: CCZ24_RS07230
• Protein ID: WP_000233000.1
• Coordinates: 1443863..1444732 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: CCZ24_RS07235
• Protein ID: WP_000205227.1
• Coordinates: 1444747..1444971 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCZ24_RS07195	1438978..1439061	-	84	Protein_1373	peptide-binding protein	-
CCZ24_RS07200	1439471..1440265	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
CCZ24_RS07205	1440358..1440609	-	252	Protein_1375	GNAT family N-acetyltransferase	-
CCZ24_RS07215	1442004..1442210	-	207	WP_044116541.1	hypothetical protein	-
CCZ24_RS07220	1442207..1443115	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
CCZ24_RS07225	1443148..1443882	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
CCZ24_RS07230	1443863..1444732	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
CCZ24_RS07235	1444747..1444971	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
CCZ24_RS11980	1445114..1445200	-	87	Protein_1382	site-specific recombinase	-
CCZ24_RS07245	1445389..1446690	+	1302	Protein_1383	ISL3 family transposase	-
CCZ24_RS07255	1446999..1447802	-	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
CCZ24_RS07260	1447856..1449340	-	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	ant(6)-Ia / erm(B) / aph(3')-III / lnu(B) / lsa(E)	cylK / cylJ / cylI / cylF / cylE / cylB / cylA / cylZ / acpC / cylG / cylD / cylX	1369674..1453748	84074
	-	inside	IScluster/Tn	ant(6)-Ia / erm(B) / aph(3')-III / lnu(B) / lsa(E)	-	1434173..1453781	19608

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T77364 WP_000233000.1 NZ_CP021773:c1444732-1443863 [Streptococcus agalactiae]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T77364 NZ_CP021773:c1444732-1443863 [Streptococcus agalactiae]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGACATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT77364 WP_000205227.1 NZ_CP021773:c1444971-1444747 [Streptococcus agalactiae]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT77364 NZ_CP021773:c1444971-1444747 [Streptococcus agalactiae]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: R3JIN1