Detailed information of TA system TA077363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 1435812..1436244
• Replicon: chromosome
• Accession: NZ_CP021773
• Organism: Streptococcus agalactiae strain B105

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: CCZ24_RS07155
• Protein ID: WP_001284310.1
• Coordinates: 1435812..1435970 (-)
• Length: 53 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: CCZ24_RS07160
• Protein ID: WP_000301765.1
• Coordinates: 1435972..1436244 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCZ24_RS07120	1431134..1432299	-	1166	Protein_1361	type IV secretory system conjugative DNA transfer family protein	-
CCZ24_RS07125	1432292..1432783	-	492	WP_000011313.1	hypothetical protein	-
CCZ24_RS07130	1432934..1433602	-	669	Protein_1363	zeta toxin family protein	-
CCZ24_RS07145	1434173..1435039	-	867	WP_001255868.1	aminoglycoside 6-adenylyltransferase AadE	-
CCZ24_RS07150	1435215..1435760	-	546	WP_001071501.1	AAA family ATPase	-
CCZ24_RS07155	1435812..1435970	-	159	WP_001284310.1	zeta toxin family protein	Toxin
CCZ24_RS07160	1435972..1436244	-	273	WP_000301765.1	antitoxin	Antitoxin
CCZ24_RS07165	1436262..1436471	-	210	WP_000527318.1	peptide-binding protein	-
CCZ24_RS07170	1436569..1437465	-	897	WP_001809760.1	ParA family protein	-
CCZ24_RS07175	1437554..1437838	-	285	WP_073459356.1	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
CCZ24_RS07185	1437984..1438721	-	738	WP_001038790.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
CCZ24_RS07190	1438846..1438929	-	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
CCZ24_RS07195	1438978..1439061	-	84	Protein_1373	peptide-binding protein	-
CCZ24_RS07200	1439471..1440265	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
CCZ24_RS07205	1440358..1440609	-	252	Protein_1375	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	ant(6)-Ia / erm(B) / aph(3')-III / lnu(B) / lsa(E)	cylK / cylJ / cylI / cylF / cylE / cylB / cylA / cylZ / acpC / cylG / cylD / cylX	1369674..1453748	84074
	-	inside	IScluster/Tn	ant(6)-Ia / erm(B) / aph(3')-III / lnu(B) / lsa(E)	-	1434173..1453781	19608

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (26-50)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5790.55 Da
• Isoelectric Point: 10.3088

>T77363 WP_001284310.1 NZ_CP021773:c1435970-1435812 [Streptococcus agalactiae]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRRR

Nucleotide

• Length: 159 bp

>T77363 NZ_CP021773:c1435970-1435812 [Streptococcus agalactiae]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGAAGAAGATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT77363 WP_000301765.1 NZ_CP021773:c1436244-1435972 [Streptococcus agalactiae]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT77363 NZ_CP021773:c1436244-1435972 [Streptococcus agalactiae]
ATGGCAGTTACGTATGAAAAAACTTTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3