Detailed information of TA system TA077362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-couple_hipB
• Location: 1425861..1427104
• Replicon: chromosome
• Accession: NZ_CP021773
• Organism: Streptococcus agalactiae strain B105

Toxin (Protein)

• Gene name: sezT
• Uniprot ID: G7SEB4
• Locus tag: CCZ24_RS07085
• Protein ID: WP_001235242.1
• Coordinates: 1425861..1426628 (-)
• Length: 256 a.a.

Antitoxin (Protein)

• Gene name: sezA
• Uniprot ID: -
• Locus tag: CCZ24_RS07090
• Protein ID: WP_000578719.1
• Coordinates: 1426628..1427104 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCZ24_RS07065	1422249..1422614	-	366	WP_000431640.1	plasmid mobilization relaxosome protein MobC	-
CCZ24_RS07070	1422617..1422979	-	363	WP_000140674.1	hypothetical protein	-
CCZ24_RS07080	1423317..1425824	-	2508	WP_001213955.1	AAA family ATPase	-
CCZ24_RS07085	1425861..1426628	-	768	WP_001235242.1	zeta toxin family protein	Toxin
CCZ24_RS07090	1426628..1427104	-	477	WP_000578719.1	helix-turn-helix domain-containing protein	Antitoxin
CCZ24_RS07095	1427174..1427464	-	291	WP_000667951.1	hypothetical protein	-
CCZ24_RS07100	1427515..1427904	-	390	WP_000048066.1	hypothetical protein	-
CCZ24_RS07105	1427901..1428128	-	228	WP_000110922.1	hypothetical protein	-
CCZ24_RS07110	1428156..1429256	-	1101	WP_000196859.1	toprim domain-containing protein	-
CCZ24_RS07115	1429320..1431011	-	1692	WP_050547274.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	ant(6)-Ia / erm(B) / aph(3')-III / lnu(B) / lsa(E)	cylK / cylJ / cylI / cylF / cylE / cylB / cylA / cylZ / acpC / cylG / cylD / cylX	1369674..1453748	84074

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 256 a.a.
• Molecular weight: 28985.80 Da
• Isoelectric Point: 5.1126

>T77362 WP_001235242.1 NZ_CP021773:c1426628-1425861 [Streptococcus agalactiae]
MRLEEFSEAEFQKALQRTIRALTRGKTIPDQPKAILLGGQSGAGKTTIHRIKQKEFQGNIIIIDGDSYRSQHPNYLALQE
KYGKDSVDYTKGFAGKMVEQLVDELSTQGYHLLIEGTLRTTQVPRQTAQLLSSKGYQVSLAVIGTKPELSYLSTLIRYEE
LYAIDPTQARATPKEHHDGIVENLVDNLRELESENLFDQIQIYQRDRTCIYDSETAEGSAAEVLQECLFGKWSKVEEEMV
KESELLLKELLEKNK

Nucleotide

• Length: 768 bp

>T77362 NZ_CP021773:c1426628-1425861 [Streptococcus agalactiae]
ATGAGACTGGAAGAATTTAGTGAGGCTGAGTTTCAGAAGGCCTTGCAGCGAACCATTCGGGCCTTAACCCGAGGAAAGAC
GATTCCAGATCAACCGAAAGCTATCTTGCTTGGTGGACAAAGCGGAGCAGGTAAGACGACTATTCATCGTATCAAGCAAA
AGGAATTTCAAGGTAATATCATTATTATCGATGGTGACAGCTACCGTTCTCAGCATCCCAATTACTTAGCCCTGCAAGAA
AAGTATGGCAAGGATAGCGTGGACTATACCAAGGGATTTGCAGGAAAAATGGTAGAACAGTTGGTTGATGAACTCAGTAC
GCAAGGCTACCATTTGCTGATTGAGGGAACCTTGCGAACTACTCAAGTTCCTCGTCAGACTGCTCAATTATTATCTTCTA
AAGGTTACCAAGTTTCCTTAGCAGTGATTGGTACCAAACCAGAGCTTTCTTATCTCAGCACCTTGATTCGTTACGAAGAG
CTTTATGCCATCGATCCGACTCAGGCTAGAGCAACACCAAAAGAACACCACGATGGGATTGTTGAGAACTTGGTTGATAA
CTTGAGGGAGTTAGAAAGTGAAAATCTCTTTGACCAGATTCAAATTTATCAAAGAGATAGAACTTGTATCTATGATTCTG
AAACTGCTGAAGGTTCCGCAGCAGAAGTGCTACAAGAATGCCTATTTGGAAAATGGAGCAAGGTAGAGGAGGAGATGGTG
AAGGAGAGTGAACTCCTACTTAAGGAATTACTTGAGAAAAATAAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18266.77 Da
• Isoelectric Point: 4.6424

>AT77362 WP_000578719.1 NZ_CP021773:c1427104-1426628 [Streptococcus agalactiae]
MIGDNIKSLRRTHDLTQPEFAKMVGISRNSLSRYENGTSTVSTELIDRICQKFNVSYVDIVGEDKMLTPVEDYHLTLKVE
VIKERGAAILSKLYRYQDSQDIVFDDESNPWILMSDDLAELINTKIYLVATFDEIERYNGYLDGIERMLDMVHHRVVA

Nucleotide

• Length: 477 bp

>AT77362 NZ_CP021773:c1427104-1426628 [Streptococcus agalactiae]
ATGATCGGAGACAATATAAAATCACTACGCCGAACACACGATTTAACACAGCCAGAATTTGCGAAAATGGTTGGGATTTC
ACGCAATAGCCTGAGTCGCTACGAAAATGGTACCAGCACTGTTTCAACTGAACTCATCGACCGTATTTGTCAGAAATTTA
ACGTTTCTTATGTCGATATTGTAGGAGAGGACAAGATGTTAACACCTGTCGAAGATTACCATCTGACTTTAAAAGTAGAA
GTGATAAAAGAACGTGGAGCAGCCATTTTATCAAAGCTTTATAGATACCAGGATAGTCAAGATATTGTTTTTGATGATGA
ATCTAATCCTTGGATTTTGATGAGTGATGACTTGGCCGAATTGATCAATACGAAAATTTACCTTGTCGCTACTTTCGATG
AAATAGAGCGTTACAATGGCTATTTAGATGGTATAGAGCGGATGTTAGACATGGTGCATCATCGGGTGGTGGCTTAA

Structures

Toxin

• AlphaFold DB: G7SEB4