Detailed information of TA system TA077351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 1877598..1878259
• Replicon: plasmid unnamed
• Accession: NZ_CP021767
• Organism: Ralstonia solanacearum strain RS 489

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A809E278
• Locus tag: CDC59_RS23300
• Protein ID: WP_064045941.1
• Coordinates: 1877846..1878259 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CDC59_RS23295
• Protein ID: WP_003278075.1
• Coordinates: 1877598..1877849 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDC59_RS23290	1873423..1877523	-	4101	WP_097909676.1	phage tail tape measure protein	-
CDC59_RS23295	1877598..1877849	+	252	WP_003278075.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CDC59_RS23300	1877846..1878259	+	414	WP_064045941.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDC59_RS23310	1878718..1879209	-	492	Protein_1449	IS5 family transposase	-
CDC59_RS23320	1879794..1880599	+	806	WP_097909006.1	IS5 family transposase	-
CDC59_RS23325	1881176..1882059	-	884	Protein_1451	IS5 family transposase	-
CDC59_RS23330	1882507..1882797	+	291	WP_097909677.1	H-NS histone family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / cyaB / flgG / fliM / fliI / acrB / adeG / cheY / cheB / cheW / motA	1..1999547	1999547

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-63)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15723.04 Da
• Isoelectric Point: 6.0853

>T77351 WP_064045941.1 NZ_CP021767:1877846-1878259 [Ralstonia solanacearum]
MSYLVDTNVLSELRRKQPDAHVLAWMQDRPRQSLYLSVLTLGEIRKGIERLDDAARRQHLIDWLETELPNYFIGRLLDID
AHTADRWGRLMSSANRPLPAIDGLLAATALQHDLILVTRNTKDFVGLDVPLINPWET

Nucleotide

• Length: 414 bp

>T77351 NZ_CP021767:1877846-1878259 [Ralstonia solanacearum]
TTGAGCTACCTGGTCGACACCAATGTCCTGTCCGAGCTACGCCGCAAGCAGCCCGATGCACACGTGCTCGCGTGGATGCA
AGACCGGCCGCGCCAGTCGCTCTACCTGAGCGTGCTGACCCTGGGCGAAATCCGCAAAGGCATCGAGCGACTGGACGACG
CAGCACGCCGCCAGCACCTGATCGACTGGCTCGAAACGGAACTGCCGAACTACTTCATCGGCCGCTTGCTCGACATCGAT
GCGCATACCGCCGATCGCTGGGGCCGGCTGATGTCGAGCGCCAACCGGCCGCTGCCGGCGATCGATGGTCTACTCGCCGC
GACCGCCCTGCAGCACGACCTCATACTGGTCACGCGTAACACGAAGGATTTCGTCGGACTCGATGTCCCACTCATCAACC
CTTGGGAAACGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9242.46 Da
• Isoelectric Point: 5.0774

>AT77351 WP_003278075.1 NZ_CP021767:1877598-1877849 [Ralstonia solanacearum]
MQSWQLQAAKARFSDVVKRAADDGPQEITVHGRPVAVVISRALFDRLSGGGESLVDFMRQSPLAGLDDVVFERERSLPRE
VEF

Nucleotide

• Length: 252 bp

>AT77351 NZ_CP021767:1877598-1877849 [Ralstonia solanacearum]
ATGCAAAGCTGGCAGTTGCAAGCCGCCAAGGCGCGGTTTTCCGACGTCGTGAAGCGCGCAGCGGACGATGGTCCGCAGGA
AATCACGGTGCATGGCCGCCCGGTCGCTGTTGTGATCTCGCGCGCACTGTTCGATCGCCTGAGTGGCGGCGGGGAGTCGC
TGGTCGACTTCATGCGCCAGTCTCCGTTGGCGGGCCTGGACGACGTCGTCTTCGAGCGCGAACGCAGCCTGCCGCGCGAG
GTCGAGTTTTGA

Structures

Toxin

• AlphaFold DB: A0A809E278