Detailed information of TA system TA077347

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/COG3657-dnstrm_HI1420
• Location: 65153..65769
• Replicon: plasmid unnamed
• Accession: NZ_CP021767
• Organism: Ralstonia solanacearum strain RS 489

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A177RF89
• Locus tag: CDC59_RS16230
• Protein ID: WP_064046021.1
• Coordinates: 65455..65769 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: CDC59_RS16225
• Protein ID: WP_003262836.1
• Coordinates: 65153..65458 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDC59_RS16205	61249..61659	+	411	WP_064046011.1	acyl-CoA thioesterase	-
CDC59_RS16210	61689..62081	+	393	WP_003262838.1	RidA family protein	-
CDC59_RS16215	62429..63019	+	591	WP_020957579.1	hypothetical protein	-
CDC59_RS16220	63618..64931	-	1314	WP_013207425.1	metabolite/H+ symporter	-
CDC59_RS16225	65153..65458	-	306	WP_003262836.1	putative addiction module antidote protein	Antitoxin
CDC59_RS16230	65455..65769	-	315	WP_064046021.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDC59_RS16235	65845..67044	-	1200	WP_064046022.1	MFS transporter	-
CDC59_RS16240	67171..67989	-	819	WP_013207428.1	GntR family transcriptional regulator	-
CDC59_RS16245	68159..68710	-	552	WP_042590730.1	helix-turn-helix transcriptional regulator	-
CDC59_RS16250	68844..69443	+	600	WP_097909313.1	GNAT family N-acetyltransferase	-
CDC59_RS16255	69704..70138	+	435	WP_042590732.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / cyaB / flgG / fliM / fliI / acrB / adeG / cheY / cheB / cheW / motA	1..1999547	1999547

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (9-98)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11885.58 Da
• Isoelectric Point: 6.7119

>T77347 WP_064046021.1 NZ_CP021767:c65769-65455 [Ralstonia solanacearum]
MLTIRTTELFDDGFCSLRDKAAQRRIQVRIDRLQMGHPGDMKAVRDGIRELRIDHGPGYRLYFVQHGVLIVLLCGGAKST
QEADSRRAIDLSRRLDVDDVDDME

Nucleotide

• Length: 315 bp

>T77347 NZ_CP021767:c65769-65455 [Ralstonia solanacearum]
ATGCTGACGATCCGCACGACCGAACTCTTTGACGACGGGTTTTGCAGCCTCCGCGACAAGGCTGCGCAACGCCGCATCCA
AGTGCGCATCGACCGCCTGCAGATGGGCCACCCGGGCGACATGAAAGCGGTACGTGACGGCATCCGCGAACTGCGGATCG
ATCATGGGCCGGGCTACCGCTTGTACTTCGTGCAGCACGGCGTCCTGATCGTACTGCTCTGCGGCGGCGCCAAGTCGACG
CAGGAAGCCGATAGCCGTCGCGCAATTGACCTGTCCCGCCGTCTCGACGTGGACGACGTGGACGACATGGAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11038.72 Da
• Isoelectric Point: 8.0667

>AT77347 WP_003262836.1 NZ_CP021767:c65458-65153 [Ralstonia solanacearum]
MTMAQRTLKTRPWDSAEHLRTEADMAAYLNACLEEAGDDPAFITHALGVVARARGMTRLARETGITREGLYKALSEDGNP
SFATMLKVIRALGIRLHAVPA

Nucleotide

• Length: 306 bp

>AT77347 NZ_CP021767:c65458-65153 [Ralstonia solanacearum]
ATGACGATGGCCCAACGCACCCTCAAGACCCGGCCGTGGGATTCGGCCGAACACCTCCGGACCGAGGCGGACATGGCCGC
CTATCTCAACGCCTGCCTGGAAGAGGCCGGGGACGACCCGGCGTTCATCACGCACGCGCTGGGCGTGGTCGCGCGTGCGC
GCGGCATGACCCGGCTCGCCCGCGAGACCGGGATCACGCGCGAAGGGCTGTACAAGGCGCTTTCCGAGGACGGCAACCCA
AGCTTTGCGACCATGCTGAAGGTCATCCGGGCGCTCGGCATCCGGCTGCACGCCGTGCCGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A177RF89