Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 75526..76049 Replicon plasmid tig00000001
Accession NZ_CP021758
Organism Klebsiella pneumoniae strain AR_0138

Toxin (Protein)


Gene name ccdB Uniprot ID V5RNQ6
Locus tag AM385_RS27455 Protein ID WP_015632464.1
Coordinates 75744..76049 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V5RQR8
Locus tag AM385_RS27450 Protein ID WP_015632465.1
Coordinates 75526..75744 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM385_RS27430 71669..72940 - 1272 WP_016162068.1 Y-family DNA polymerase -
AM385_RS27435 72940..73371 - 432 WP_015632467.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
AM385_RS27440 73535..74020 - 486 Protein_74 IS5 family transposase -
AM385_RS27445 74315..75340 - 1026 WP_015632466.1 IS110 family transposase -
AM385_RS27450 75526..75744 + 219 WP_015632465.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AM385_RS27455 75744..76049 + 306 WP_015632464.1 type II toxin-antitoxin system toxin CcdB Toxin
AM385_RS27460 76194..76538 + 345 WP_015632463.1 hypothetical protein -
AM385_RS27465 76652..77146 + 495 WP_161537194.1 IS1 family transposase -
AM385_RS27470 77350..77607 - 258 WP_016162071.1 helix-turn-helix domain-containing protein -
AM385_RS27475 78037..78264 + 228 WP_016162072.1 hypothetical protein -
AM385_RS27480 78278..78400 + 123 Protein_82 transcriptional regulator -
AM385_RS27485 78606..79049 - 444 WP_015632459.1 type II toxin-antitoxin system HicB family antitoxin -
AM385_RS27490 79075..79257 - 183 WP_015632458.1 type II toxin-antitoxin system HicA family toxin -
AM385_RS27495 79618..80598 + 981 WP_015632457.1 partitioning protein -
AM385_RS27500 80591..81019 + 429 WP_015632456.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ARR-3 / rmtF - 1..121057 121057


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11361.90 Da        Isoelectric Point: 5.5252

>T77340 WP_015632464.1 NZ_CP021758:75744-76049 [Klebsiella pneumoniae]
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI

Download         Length: 306 bp

>T77340 NZ_CP021758:75744-76049 [Klebsiella pneumoniae]
ATGCAGTTCACCGTCTACGAATACACACGCGAGAGCCATTATCGTCTTTTTGTGGATGTGCAGAGCGACATTGTCGAGAC
GCCTGGGCGACGCATAGTGATCCCGTTGCTGGAATCGCACCACTTATCAGAAAAGGTTAATCAGCAGCTTTTCCCGGTGG
TTAGCGTCAATGGGAAAGAATACCGTGCCGTGACTTCCGAATTATCCGGAGTCTCTGTCAGTGTCATCGGCGACGCTGTG
GCCGATCTCAGCCATGAGGCAAATGCCATTAAAAATGCCCTGAACCTGATGTTCTGGGGCATTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8194.13 Da        Isoelectric Point: 6.2224

>AT77340 WP_015632465.1 NZ_CP021758:75526-75744 [Klebsiella pneumoniae]
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW

Download         Length: 219 bp

>AT77340 NZ_CP021758:75526-75744 [Klebsiella pneumoniae]
ATGAAACACAGAGCCAGCGTCACAGTAGACAAGGAACATTATCTGGAATTACTCGCGGCAGGCGTGAATATTTCGGGACT
GGTGAATGAGGCTATCGGACGGGAAGCCCGGCGCATCCAGGCTGAACGCTGGAAAGCAGAGAACCGGGAAGGGATGGAAG
AGGTAGCGAATTTCATCGCGCAGCACGGCTCTTTTGCCGACGAAAACCGGAGCTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0ND19

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0L1T7

References