Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1669415..1670151 Replicon chromosome
Accession NZ_CP021757
Organism Klebsiella pneumoniae strain AR_0138

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag AM385_RS08385 Protein ID WP_032433360.1
Coordinates 1669415..1669897 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AM385_RS08390 Protein ID WP_003026799.1
Coordinates 1669885..1670151 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM385_RS08365 1665964..1666704 + 741 WP_050597964.1 molecular chaperone -
AM385_RS08370 1666708..1667286 + 579 WP_032432061.1 type 1 fimbrial protein -
AM385_RS08375 1667298..1668242 + 945 WP_077254249.1 fimbrial protein -
AM385_RS08380 1668455..1669054 + 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
AM385_RS08385 1669415..1669897 - 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
AM385_RS08390 1669885..1670151 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AM385_RS08395 1670464..1671027 - 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
AM385_RS08400 1671037..1671669 - 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
AM385_RS08405 1671687..1672691 - 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
AM385_RS08410 1672675..1673337 - 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
AM385_RS08415 1673366..1674505 - 1140 WP_032433368.1 mannitol dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1659645..1702206 42561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T77330 WP_032433360.1 NZ_CP021757:c1669897-1669415 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T77330 NZ_CP021757:c1669897-1669415 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT77330 WP_003026799.1 NZ_CP021757:c1670151-1669885 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT77330 NZ_CP021757:c1670151-1669885 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References