Detailed information of TA system TA077319

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 43119..43608
• Replicon: plasmid unitig_3
• Accession: NZ_CP021754
• Organism: Klebsiella pneumoniae strain AR_0113

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U3PL64
• Locus tag: AM360_RS29455
• Protein ID: WP_020314676.1
• Coordinates: 43119..43379 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: AM360_RS29460
• Protein ID: WP_022540736.1
• Coordinates: 43369..43608 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM360_RS29405	39207..39536	+	330	WP_001542022.1	hypothetical protein	-
AM360_RS29410	39940..40455	+	516	WP_001025393.1	J domain-containing protein	-
AM360_RS29415	40559..41101	+	543	WP_001083904.1	hypothetical protein	-
AM360_RS29420	41098..41352	+	255	WP_001230709.1	hypothetical protein	-
AM360_RS29425	41349..41591	+	243	WP_000151456.1	hypothetical protein	-
AM360_RS29440	42172..42495	+	324	WP_020314677.1	hypothetical protein	-
AM360_RS29445	42537..42746	+	210	WP_020314678.1	hypothetical protein	-
AM360_RS29450	42843..43067	+	225	WP_000814381.1	hypothetical protein	-
AM360_RS29455	43119..43379	+	261	WP_020314676.1	hypothetical protein	Toxin
AM360_RS29460	43369..43608	+	240	WP_022540736.1	transcriptional regulator	Antitoxin
AM360_RS29475	44143..44403	+	261	WP_020314977.1	hypothetical protein	-
AM360_RS29480	44790..44972	-	183	WP_001401700.1	hypothetical protein	-
AM360_RS29485	45517..45699	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
AM360_RS29490	45724..46161	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
AM360_RS29495	46292..46612	-	321	WP_001310435.1	hypothetical protein	-
AM360_RS29500	46609..47124	-	516	WP_001229189.1	hypothetical protein	-
AM360_RS29505	47161..47424	-	264	WP_020314988.1	hypothetical protein	-
AM360_RS29510	47426..47845	-	420	WP_001708405.1	hypothetical protein	-
AM360_RS29515	47865..48572	-	708	WP_000108796.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-3 / blaTEM-1A	-	1..74751	74751

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9922.30 Da
• Isoelectric Point: 7.2028

>T77319 WP_020314676.1 NZ_CP021754:43119-43379 [Klebsiella pneumoniae]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRDNDKEIVFMMIQEMS
EEENAN

Nucleotide

• Length: 261 bp

>T77319 NZ_CP021754:43119-43379 [Klebsiella pneumoniae]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGATAATGATAAAGAAATTGTTTTTATGATGATTCAGGAAATGAGC
GAAGAAGAAAATGCTAACTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9072.61 Da
• Isoelectric Point: 10.2613

>AT77319 WP_022540736.1 NZ_CP021754:43369-43608 [Klebsiella pneumoniae]
MLTENTPENIKRLRKKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKYLLLLAGEHPEYVLSKRDRGILK

Nucleotide

• Length: 240 bp

>AT77319 NZ_CP021754:43369-43608 [Klebsiella pneumoniae]
ATGCTAACTGAAAACACACCAGAAAACATTAAGCGTCTGCGGAAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTCAGCATGAGTCCGCGTACATGGCGTCGTAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCCGTGG
AGTTTAAATATCTTCTGCTTTTAGCTGGGGAACATCCTGAATACGTCCTTAGTAAAAGAGATCGAGGAATTTTAAAATGA

Structures

Toxin

• AlphaFold DB: A0A376MG86