Detailed information of TA system TA077318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 41098..41591
• Replicon: plasmid unitig_3
• Accession: NZ_CP021754
• Organism: Klebsiella pneumoniae strain AR_0113

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: AM360_RS29420
• Protein ID: WP_001230709.1
• Coordinates: 41098..41352 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: AM360_RS29425
• Protein ID: WP_000151456.1
• Coordinates: 41349..41591 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM360_RS29380	36672..36833	+	162	WP_001434039.1	type I toxin-antitoxin system Hok family toxin	-
AM360_RS29385	36983..37579	+	597	WP_000125164.1	hypothetical protein	-
AM360_RS30990	37597..37881	-	285	WP_001162371.1	hypothetical protein	-
AM360_RS29395	37955..38620	-	666	WP_000532148.1	P-loop NTPase	-
AM360_RS29405	39207..39536	+	330	WP_001542022.1	hypothetical protein	-
AM360_RS29410	39940..40455	+	516	WP_001025393.1	J domain-containing protein	-
AM360_RS29415	40559..41101	+	543	WP_001083904.1	hypothetical protein	-
AM360_RS29420	41098..41352	+	255	WP_001230709.1	hypothetical protein	Toxin
AM360_RS29425	41349..41591	+	243	WP_000151456.1	hypothetical protein	Antitoxin
AM360_RS29440	42172..42495	+	324	WP_020314677.1	hypothetical protein	-
AM360_RS29445	42537..42746	+	210	WP_020314678.1	hypothetical protein	-
AM360_RS29450	42843..43067	+	225	WP_000814381.1	hypothetical protein	-
AM360_RS29455	43119..43379	+	261	WP_020314676.1	hypothetical protein	-
AM360_RS29460	43369..43608	+	240	WP_022540736.1	transcriptional regulator	-
AM360_RS29475	44143..44403	+	261	WP_020314977.1	hypothetical protein	-
AM360_RS29480	44790..44972	-	183	WP_001401700.1	hypothetical protein	-
AM360_RS29485	45517..45699	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
AM360_RS29490	45724..46161	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-3 / blaTEM-1A	-	1..74751	74751

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T77318 WP_001230709.1 NZ_CP021754:41098-41352 [Klebsiella pneumoniae]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T77318 NZ_CP021754:41098-41352 [Klebsiella pneumoniae]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT77318 WP_000151456.1 NZ_CP021754:41349-41591 [Klebsiella pneumoniae]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT77318 NZ_CP021754:41349-41591 [Klebsiella pneumoniae]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5