Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 85249..85774 Replicon plasmid unitig_2
Accession NZ_CP021753
Organism Klebsiella pneumoniae strain AR_0113

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag AM360_RS28975 Protein ID WP_001568026.1
Coordinates 85469..85774 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag AM360_RS28970 Protein ID WP_001568025.1
Coordinates 85249..85467 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM360_RS28925 80626..80829 - 204 WP_004197809.1 hypothetical protein -
AM360_RS28930 80843..81046 - 204 WP_004197808.1 hemolysin expression modulator Hha -
AM360_RS28935 81080..81448 - 369 WP_004197807.1 hypothetical protein -
AM360_RS28940 81492..81986 - 495 WP_020802777.1 hypothetical protein -
AM360_RS28945 82017..82592 - 576 WP_020317495.1 hypothetical protein -
AM360_RS28950 82580..82849 - 270 WP_020804424.1 hypothetical protein -
AM360_RS28955 83286..83975 + 690 WP_020315560.1 RES family NAD+ phosphorylase -
AM360_RS28960 84007..84696 - 690 WP_004193995.1 hypothetical protein -
AM360_RS28970 85249..85467 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AM360_RS28975 85469..85774 + 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
AM360_RS28980 85943..86338 + 396 WP_017899885.1 hypothetical protein -
AM360_RS28985 86365..86688 + 324 WP_022644730.1 hypothetical protein -
AM360_RS28990 86685..87701 + 1017 WP_020315256.1 hypothetical protein -
AM360_RS28995 87899..88693 + 795 WP_004197635.1 site-specific integrase -
AM360_RS29000 89149..89328 - 180 WP_006788217.1 hypothetical protein -
AM360_RS29005 89448..90074 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..116187 116187


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T77317 WP_001568026.1 NZ_CP021753:85469-85774 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T77317 NZ_CP021753:85469-85774 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT77317 WP_001568025.1 NZ_CP021753:85249-85467 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT77317 NZ_CP021753:85249-85467 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References