Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA(toxin)
Location 4536823..4538606 Replicon chromosome
Accession NZ_CP021749
Organism Enterobacter cloacae complex sp. strain AR_0163

Toxin (Protein)


Gene name hipA Uniprot ID A0A2G5A822
Locus tag AM409_RS22430 Protein ID WP_045333653.1
Coordinates 4537257..4538606 (-) Length 450 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A2G5A843
Locus tag AM409_RS22425 Protein ID WP_045333654.1
Coordinates 4536823..4537260 (-) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM409_RS22405 (AM409_22290) 4531842..4532864 + 1023 WP_003859324.1 HTH-type transcriptional regulator GalS -
AM409_RS22410 (AM409_22295) 4533160..4534158 + 999 WP_017383382.1 galactose/glucose ABC transporter substrate-binding protein MglB -
AM409_RS22415 (AM409_22300) 4534238..4535758 + 1521 WP_047727290.1 galactose/methyl galactoside ABC transporter ATP-binding protein MglA -
AM409_RS22420 (AM409_22305) 4535774..4536784 + 1011 WP_003859318.1 galactose/methyl galactoside ABC transporter permease MglC -
AM409_RS22425 (AM409_22310) 4536823..4537260 - 438 WP_045333654.1 helix-turn-helix transcriptional regulator Antitoxin
AM409_RS22430 (AM409_22315) 4537257..4538606 - 1350 WP_045333653.1 HipA domain-containing protein Toxin
AM409_RS22435 (AM409_22320) 4538741..4539451 - 711 WP_017383381.1 outer membrane permeability protein SanA -
AM409_RS22440 (AM409_22325) 4539579..4540463 - 885 WP_017383380.1 cytidine deaminase -
AM409_RS22445 (AM409_22330) 4540592..4541287 - 696 WP_015572322.1 CidB/LrgB family autolysis modulator -
AM409_RS22450 (AM409_22335) 4541284..4541679 - 396 WP_003859308.1 CidA/LrgA family protein -
AM409_RS22455 (AM409_22340) 4541848..4542783 + 936 WP_015572323.1 tRNA dihydrouridine(16) synthase DusC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(166-406)

Antitoxin

Cro/C1-type helix-turn-helix domain

(75-128)


Sequences

Toxin        


Download         Length: 450 a.a.        Molecular weight: 51197.84 Da        Isoelectric Point: 4.6562

>T77290 WP_045333653.1 NZ_CP021749:c4538606-4537257 [Enterobacter cloacae complex sp.]
METLTVQAYLNETWTDIALIKYPGSEKNDWNTTQLDYLTEYAINFLDYDDFHAVSVNHPVSLFFDDHGQPGWLRFIDDII
PAGASRRYWINALDISELPVGQQNFLLLKFGTMSPVGNLRIKESVPEWNKFASTKTFTVADVIDRAADFLDYAQERGAAA
GGATGAGGEAPKLLLRCSETDAIWIDTWQNEPDNRDRYYLVKYPRGNRTEVDCNILRAEFHYYHELNAMGFSTIPVETMR
LEEGSHYPSLWLPRFDIVRNEQDQLVRLGMESVYSLLQKAPGTLLDHETTLRTLINKITSSHMVQQQGYHFDIPGFVTEW
VRRDLLNIIFGNSDNHGRNTAFVKAENQIMLSPVYDFAPMKADPEGIPRTLKWSLSCESGGDYNFNTIAQTLAEWIPPTT
LLDALHETAVQLIDLPERLAARGVPEQIMEMPAMGFRYIPDKLSRWGLL

Download         Length: 1350 bp

>T77290 NZ_CP021749:c4538606-4537257 [Enterobacter cloacae complex sp.]
ATGGAGACGTTAACCGTACAGGCATATCTGAACGAAACATGGACGGATATCGCTCTCATCAAATATCCAGGAAGTGAAAA
AAATGACTGGAATACCACGCAGTTAGACTATCTCACTGAGTATGCGATTAATTTTCTCGACTATGATGATTTTCACGCAG
TCTCAGTCAATCACCCTGTATCACTCTTTTTTGATGACCATGGCCAACCGGGCTGGCTGCGATTTATCGACGATATTATT
CCCGCCGGAGCAAGCCGCCGTTACTGGATTAATGCGCTCGACATCAGTGAATTACCCGTTGGCCAGCAAAATTTCTTATT
GCTGAAATTTGGCACAATGTCCCCTGTGGGGAATTTGCGCATAAAAGAATCCGTCCCAGAATGGAATAAATTCGCCAGCA
CTAAAACGTTTACGGTTGCGGACGTAATCGATCGTGCGGCCGATTTTCTGGATTATGCCCAGGAAAGAGGCGCGGCGGCG
GGTGGTGCAACCGGTGCTGGCGGTGAAGCGCCTAAACTTTTGCTGCGCTGTAGTGAAACTGATGCCATCTGGATCGATAC
CTGGCAAAACGAGCCGGATAACCGGGACCGCTATTATTTGGTTAAATATCCACGCGGTAACAGGACAGAAGTTGATTGTA
ATATTCTGCGAGCTGAATTCCATTATTATCATGAGCTTAACGCGATGGGTTTTTCCACGATCCCAGTGGAAACAATGCGC
CTTGAAGAAGGTAGCCATTACCCGTCTCTGTGGTTACCGCGTTTCGACATAGTGAGAAATGAGCAGGACCAACTGGTCAG
GCTGGGTATGGAATCGGTGTATTCGCTATTACAAAAGGCACCCGGCACCCTCCTTGATCATGAGACAACGTTGCGCACGT
TAATTAATAAAATCACCTCCAGCCATATGGTCCAGCAGCAGGGCTATCATTTTGATATTCCGGGATTTGTAACAGAATGG
GTGCGTCGCGATCTGCTAAATATTATTTTTGGAAATAGTGACAACCATGGGCGAAATACCGCCTTTGTGAAGGCTGAAAA
TCAAATTATGCTCTCCCCTGTATATGACTTTGCGCCGATGAAAGCCGATCCTGAAGGGATACCAAGAACCCTGAAGTGGT
CATTATCTTGCGAATCAGGAGGGGATTATAATTTCAACACTATCGCACAAACGCTGGCGGAGTGGATCCCTCCCACAACA
TTACTTGATGCTCTGCATGAAACAGCCGTTCAGCTTATTGATCTCCCGGAAAGGCTGGCGGCGCGAGGCGTTCCGGAACA
GATAATGGAAATGCCCGCGATGGGTTTTCGCTACATCCCGGATAAACTTTCCCGTTGGGGTTTATTATGA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16171.61 Da        Isoelectric Point: 10.5154

>AT77290 WP_045333654.1 NZ_CP021749:c4537260-4536823 [Enterobacter cloacae complex sp.]
MKTPNEAESELLQTLAQVRSVNKKRHHDEAEDPDKPSVVKRQRVTQGLTRISTLDRQAVLHAAIRDILLGKITQGEALKR
LRVEVLGLKQDEYAKLVSVSRKTLSDVENNRGNYSADVINKIFKPFGLQTGLVPVSKSLIASLFS

Download         Length: 438 bp

>AT77290 NZ_CP021749:c4537260-4536823 [Enterobacter cloacae complex sp.]
ATGAAAACACCTAATGAAGCTGAATCAGAGCTTTTACAGACATTAGCTCAAGTACGCAGCGTAAATAAAAAGCGACATCA
TGACGAGGCGGAAGATCCGGACAAGCCATCGGTAGTGAAGAGGCAACGCGTTACACAAGGCTTAACGCGGATTAGTACTC
TTGATCGCCAGGCTGTACTGCATGCAGCCATACGGGACATTTTGCTGGGGAAAATTACACAGGGAGAGGCGCTGAAAAGG
CTCAGGGTCGAGGTGTTAGGGCTGAAGCAGGATGAATATGCAAAGCTGGTCAGCGTATCCCGGAAAACATTGTCGGATGT
AGAAAATAACAGAGGCAATTATTCCGCTGACGTCATAAATAAAATCTTTAAACCCTTTGGGCTTCAAACCGGACTAGTGC
CCGTTTCAAAATCGCTTATCGCTTCACTTTTCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G5A822

Antitoxin

Source ID Structure
AlphaFold DB A0A2G5A843

References