Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 2710866..2711470 Replicon chromosome
Accession NZ_CP021749
Organism Enterobacter cloacae complex sp. strain AR_0163

Toxin (Protein)


Gene name hicA Uniprot ID A0A2J0PXG2
Locus tag AM409_RS13205 Protein ID WP_071788668.1
Coordinates 2710866..2711051 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2G4ZRB5
Locus tag AM409_RS13210 Protein ID WP_023295606.1
Coordinates 2711066..2711470 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM409_RS13190 (AM409_13130) 2706185..2708023 + 1839 WP_045911843.1 selenocysteine-specific translation elongation factor -
AM409_RS13195 (AM409_13135) 2708027..2708911 - 885 WP_045911842.1 ROK family protein -
AM409_RS13200 (AM409_13140) 2709078..2710616 + 1539 WP_045911841.1 aldehyde dehydrogenase AldB -
AM409_RS13205 (AM409_13145) 2710866..2711051 + 186 WP_071788668.1 type II toxin-antitoxin system HicA family toxin Toxin
AM409_RS13210 (AM409_13150) 2711066..2711470 + 405 WP_023295606.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AM409_RS13215 (AM409_13155) 2711526..2713475 - 1950 WP_045911840.1 glycoside hydrolase family 127 protein -
AM409_RS13220 (AM409_13160) 2713486..2714886 - 1401 WP_045142691.1 MFS transporter -
AM409_RS13225 (AM409_13165) 2715112..2715930 + 819 WP_045911839.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6822.05 Da        Isoelectric Point: 11.5191

>T77286 WP_071788668.1 NZ_CP021749:2710866-2711051 [Enterobacter cloacae complex sp.]
VKSADIIAVLVSHGWKCVRTKGSHHQFRHPVQKGLVTVPHPKKDIKPGTLAQIWRQAGIKH

Download         Length: 186 bp

>T77286 NZ_CP021749:2710866-2711051 [Enterobacter cloacae complex sp.]
GTGAAAAGTGCGGATATCATTGCTGTTCTGGTTAGCCATGGCTGGAAATGTGTAAGAACGAAGGGAAGCCATCATCAGTT
TCGCCATCCAGTGCAAAAAGGGTTGGTAACCGTTCCGCATCCTAAAAAGGACATTAAACCGGGCACTCTCGCACAGATCT
GGCGACAGGCGGGCATTAAACACTGA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14954.71 Da        Isoelectric Point: 4.2329

>AT77286 WP_023295606.1 NZ_CP021749:2711066-2711470 [Enterobacter cloacae complex sp.]
MFYPAYIHSDSDGSASGFFPDVPGCFFAGDSLDDAFQDARDALTAHFEALFEMDEALPLPGSVEVHLENQPDDFTGGQWL
LVDINMKQFDGRAERINITMPRRLLVKIDSFVSEHPQFGNRSAFLAEAARRVLP

Download         Length: 405 bp

>AT77286 NZ_CP021749:2711066-2711470 [Enterobacter cloacae complex sp.]
ATGTTTTATCCGGCTTACATTCATTCTGATTCCGACGGTAGCGCCAGCGGTTTCTTCCCCGACGTTCCGGGATGTTTTTT
TGCGGGGGATTCACTTGATGACGCTTTTCAGGATGCACGTGATGCGCTCACGGCGCACTTCGAAGCGCTGTTTGAAATGG
ATGAAGCATTGCCTCTCCCGGGTAGTGTCGAAGTGCACCTTGAAAATCAACCTGACGACTTCACAGGCGGGCAATGGTTG
CTGGTGGATATTAATATGAAGCAATTCGACGGCAGAGCCGAACGCATCAACATCACGATGCCTCGACGTTTGCTGGTGAA
GATTGATTCCTTTGTCAGCGAGCATCCTCAATTCGGCAATCGAAGCGCATTTCTGGCCGAGGCAGCACGTCGCGTGCTGC
CGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0PXG2

Antitoxin

Source ID Structure
AlphaFold DB A0A2G4ZRB5

References