Detailed information of TA system TA077285

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2587170..2587786
• Replicon: chromosome
• Accession: NZ_CP021749
• Organism: Enterobacter cloacae complex sp. strain AR_0163

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AM409_RS12645
• Protein ID: WP_077266491.1
• Coordinates: 2587415..2587786 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: AM409_RS12640
• Protein ID: WP_015569912.1
• Coordinates: 2587170..2587412 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM409_RS12610 (AM409_12545)	2582229..2583029	+	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-
AM409_RS12615 (AM409_12550)	2583148..2583747	+	600	WP_003861947.1	glucose-1-phosphatase	-
AM409_RS12620 (AM409_12555)	2583741..2584622	+	882	WP_003861949.1	virulence factor BrkB family protein	-
AM409_RS12625 (AM409_12560)	2584619..2585056	+	438	WP_015569909.1	D-aminoacyl-tRNA deacylase	-
AM409_RS12630 (AM409_12565)	2585101..2586042	+	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
AM409_RS12635 (AM409_12570)	2586051..2586971	-	921	WP_017382675.1	alpha/beta hydrolase	-
AM409_RS12640 (AM409_12575)	2587170..2587412	+	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
AM409_RS12645 (AM409_12580)	2587415..2587786	+	372	WP_077266491.1	type II toxin-antitoxin system VapC family toxin	Toxin
AM409_RS12650 (AM409_12585)	2587826..2588755	-	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
AM409_RS12655 (AM409_12590)	2588752..2589387	-	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
AM409_RS12660 (AM409_12595)	2589384..2590286	-	903	WP_014072386.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13723.85 Da
• Isoelectric Point: 6.4882

>T77285 WP_077266491.1 NZ_CP021749:2587415-2587786 [Enterobacter cloacae complex sp.]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T77285 NZ_CP021749:2587415-2587786 [Enterobacter cloacae complex sp.]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT77285 WP_015569912.1 NZ_CP021749:2587170-2587412 [Enterobacter cloacae complex sp.]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT77285 NZ_CP021749:2587170-2587412 [Enterobacter cloacae complex sp.]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAACTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3