Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1316584..1317204 Replicon chromosome
Accession NZ_CP021749
Organism Enterobacter cloacae complex sp. strain AR_0163

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag AM409_RS06490 Protein ID WP_015571250.1
Coordinates 1316986..1317204 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag AM409_RS06485 Protein ID WP_006809850.1
Coordinates 1316584..1316958 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM409_RS06475 (AM409_06425) 1311710..1312903 + 1194 WP_017694395.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AM409_RS06480 (AM409_06430) 1312926..1316072 + 3147 WP_015571248.1 multidrug efflux RND transporter permease subunit AcrB -
AM409_RS06485 (AM409_06435) 1316584..1316958 + 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
AM409_RS06490 (AM409_06440) 1316986..1317204 + 219 WP_015571250.1 HHA domain-containing protein Toxin
AM409_RS06495 (AM409_06445) 1317413..1317964 + 552 WP_015571251.1 maltose O-acetyltransferase -
AM409_RS06500 (AM409_06450) 1318081..1318548 + 468 WP_023296041.1 YlaC family protein -
AM409_RS06505 (AM409_06455) 1318520..1319980 - 1461 WP_087924301.1 PLP-dependent aminotransferase family protein -
AM409_RS06510 (AM409_06460) 1320082..1320792 + 711 WP_032670463.1 GNAT family protein -
AM409_RS06515 (AM409_06465) 1320789..1320929 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
AM409_RS06520 (AM409_06470) 1320932..1321192 - 261 WP_015571255.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T77284 WP_015571250.1 NZ_CP021749:1316986-1317204 [Enterobacter cloacae complex sp.]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T77284 NZ_CP021749:1316986-1317204 [Enterobacter cloacae complex sp.]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTTGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAGC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTGTGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT77284 WP_006809850.1 NZ_CP021749:1316584-1316958 [Enterobacter cloacae complex sp.]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT77284 NZ_CP021749:1316584-1316958 [Enterobacter cloacae complex sp.]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTCAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References