Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 18680..19205 Replicon plasmid tig00000001
Accession NZ_CP021741
Organism Klebsiella pneumoniae strain AR_0126

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag AM373_RS27115 Protein ID WP_004197633.1
Coordinates 18900..19205 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag AM373_RS27110 Protein ID WP_006788213.1
Coordinates 18680..18898 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM373_RS27070 13970..14264 + 295 Protein_18 korC -
AM373_RS27080 14853..15854 + 1002 WP_009483873.1 DUF4238 domain-containing protein -
AM373_RS27085 16039..16383 - 345 WP_009483875.1 hypothetical protein -
AM373_RS27090 16870..17160 - 291 WP_023287115.1 hypothetical protein -
AM373_RS27095 17204..18127 - 924 WP_009483877.1 hypothetical protein -
AM373_RS27100 18283..18510 + 228 Protein_23 hypothetical protein -
AM373_RS27110 18680..18898 + 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AM373_RS27115 18900..19205 + 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
AM373_RS28615 19263..19574 + 312 WP_023287114.1 hypothetical protein -
AM373_RS27120 19624..19959 + 336 WP_009309920.1 hypothetical protein -
AM373_RS27125 19993..21009 + 1017 WP_009483880.1 hypothetical protein -
AM373_RS29140 21053..21214 + 162 WP_009483881.1 hypothetical protein -
AM373_RS27130 21207..21986 + 780 WP_023287113.1 site-specific integrase -
AM373_RS27135 22044..22301 - 258 WP_023287112.1 hypothetical protein -
AM373_RS29145 22430..22543 - 114 WP_014343462.1 hypothetical protein -
AM373_RS27140 23079..23945 + 867 WP_004118283.1 replication initiation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA1 / ant(3'')-Ia / qacE / sul1 - 1..89621 89621


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T77279 WP_004197633.1 NZ_CP021741:18900-19205 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T77279 NZ_CP021741:18900-19205 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT77279 WP_006788213.1 NZ_CP021741:18680-18898 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT77279 NZ_CP021741:18680-18898 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References