Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 65516..66041 Replicon plasmid tig00000255
Accession NZ_CP021737
Organism Escherichia coli strain AR_0150

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag AM397_RS25385 Protein ID WP_001159871.1
Coordinates 65736..66041 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag AM397_RS25380 Protein ID WP_000813630.1
Coordinates 65516..65734 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM397_RS25340 60726..61412 + 687 Protein_65 IS1 family transposase -
AM397_RS25350 61666..62688 - 1023 WP_000361402.1 helicase UvrD -
AM397_RS25355 62673..64238 - 1566 WP_001128474.1 AAA family ATPase -
AM397_RS25360 64313..64729 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
AM397_RS25365 64726..64956 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
AM397_RS25380 65516..65734 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AM397_RS25385 65736..66041 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
AM397_RS25390 66042..66848 + 807 WP_000016970.1 site-specific integrase -
AM397_RS25395 67570..68325 + 756 WP_000852146.1 replication initiation protein RepE -
AM397_RS25400 68913..70079 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / dfrA17 / aadA5 / qacE / sul1 / mph(A) - 1..117703 117703


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T77254 WP_001159871.1 NZ_CP021737:65736-66041 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T77254 NZ_CP021737:65736-66041 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT77254 WP_000813630.1 NZ_CP021737:65516-65734 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT77254 NZ_CP021737:65516-65734 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References