Detailed information of TA system TA077241

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4232347..4232985
• Replicon: chromosome
• Accession: NZ_CP021736
• Organism: Escherichia coli strain AR_0150

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: AM397_RS21680
• Protein ID: WP_001447010.1
• Coordinates: 4232347..4232523 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AM397_RS21685
• Protein ID: WP_001270286.1
• Coordinates: 4232569..4232985 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM397_RS21660	4227966..4229180	-	1215	WP_001326689.1	BenE family transporter YdcO	-
AM397_RS21665	4229233..4229769	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
AM397_RS21670	4229842..4231803	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AM397_RS21675	4231895..4232125	-	231	WP_000494244.1	YncJ family protein	-
AM397_RS21680	4232347..4232523	+	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AM397_RS21685	4232569..4232985	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AM397_RS21690	4233064..4234470	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
AM397_RS21695	4234715..4235860	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
AM397_RS21700	4235878..4236891	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
AM397_RS21705	4236892..4237833	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T77241 WP_001447010.1 NZ_CP021736:4232347-4232523 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T77241 NZ_CP021736:4232347-4232523 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT77241 WP_001270286.1 NZ_CP021736:4232569-4232985 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT77241 NZ_CP021736:4232569-4232985 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8