Detailed information of TA system TA077239

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3015874..3016568
• Replicon: chromosome
• Accession: NZ_CP021736
• Organism: Escherichia coli strain AR_0150

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: AM397_RS15360
• Protein ID: WP_001094398.1
• Coordinates: 3016200..3016568 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: AM397_RS15355
• Protein ID: WP_077249034.1
• Coordinates: 3015874..3016179 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM397_RS15315	3011824..3012279	+	456	WP_000581506.1	hypothetical protein	-
AM397_RS26575	3012380..3012588	+	209	Protein_2782	DUF905 family protein	-
AM397_RS15325	3012689..3013510	+	822	WP_001234565.1	DUF945 domain-containing protein	-
AM397_RS15335	3013852..3014325	+	474	WP_000855059.1	antirestriction protein	-
AM397_RS15340	3014341..3014817	+	477	WP_001424026.1	RadC family protein	-
AM397_RS15345	3014886..3015107	+	222	WP_000692345.1	DUF987 domain-containing protein	-
AM397_RS15350	3015126..3015770	+	645	WP_000086755.1	hypothetical protein	-
AM397_RS15355	3015874..3016179	+	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM397_RS15360	3016200..3016568	+	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
AM397_RS15365	3016889..3017227	-	339	Protein_2790	LysR family transcriptional regulator	-
AM397_RS15370	3017278..3018234	-	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
AM397_RS15375	3018244..3020442	-	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
AM397_RS15380	3020439..3021395	-	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3000215..3023113	22898

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T77239 WP_001094398.1 NZ_CP021736:3016200-3016568 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T77239 NZ_CP021736:3016200-3016568 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT77239 WP_077249034.1 NZ_CP021736:3015874-3016179 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT77239 NZ_CP021736:3015874-3016179 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4