Detailed information of TA system TA077221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 73738..74168
• Replicon: plasmid unitig_1
• Accession: NZ_CP021733
• Organism: Escherichia coli strain AR_0114

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: AM361_RS26480
• Protein ID: WP_001388374.1
• Coordinates: 73738..73902 (-)
• Length: 55 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A6Y0EDY2
• Locus tag: AM361_RS25075
• Protein ID: WP_000079939.1
• Coordinates: 73899..74168 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM361_RS25040	68807..69622	+	816	WP_001043260.1	sulfonamide-resistant dihydropteroate synthase Sul2	-
AM361_RS25045	69683..70486	+	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-
AM361_RS25050	70486..71322	+	837	WP_000480972.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
AM361_RS25055	71429..71833	-	405	Protein_94	IS91 family transposase	-
AM361_RS25060	71945..72325	-	381	WP_001696636.1	HTH domain-containing protein	-
AM361_RS25070	72767..73690	-	924	WP_001415483.1	hypothetical protein	-
AM361_RS26480	73738..73902	-	165	WP_001388374.1	hypothetical protein	Toxin
AM361_RS25075	73899..74168	-	270	WP_000079939.1	hypothetical protein	Antitoxin
AM361_RS26665	74241..74381	-	141	WP_001404367.1	hypothetical protein	-
AM361_RS25090	75084..75941	-	858	WP_048266445.1	incFII family plasmid replication initiator RepA	-
AM361_RS25095	75934..76008	-	75	WP_001365705.1	RepA leader peptide Tap	-
AM361_RS25105	76243..76500	-	258	WP_000083821.1	replication regulatory protein RepA	-
AM361_RS25110	76905..77927	+	1023	WP_000156883.1	IS110 family transposase	-
AM361_RS25115	78399..78752	+	354	WP_000005489.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / sul1 / qacE / cmlA1 / ant(3'')-Ia / ant(2'')-Ia / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id	-	1..114267	114267

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 55 a.a.
• Molecular weight: 6604.73 Da
• Isoelectric Point: 7.1989

>T77221 WP_001388374.1 NZ_CP021733:c73902-73738 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYFGRTHRLTPVLHLYYM

Nucleotide

• Length: 165 bp

>T77221 NZ_CP021733:c73902-73738 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAATACATTGCGGAGCAGAACCTGAT
AGCAGCCATTGAACTGGACGAACGGATTGGTTATTTCGGACGAACGCACCGGCTTACGCCGGTTTTACATCTGTACTACA
TGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10233.54 Da
• Isoelectric Point: 6.7263

>AT77221 WP_000079939.1 NZ_CP021733:c74168-73899 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT77221 NZ_CP021733:c74168-73899 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGTCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCACGGCGATGTACTTCCCCATGACATGGTCGAATCCTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6Y0EDY2