Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 34212..34855 Replicon plasmid unitig_1
Accession NZ_CP021733
Organism Escherichia coli strain AR_0114

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag AM361_RS24795 Protein ID WP_001034044.1
Coordinates 34439..34855 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag AM361_RS24790 Protein ID WP_001261286.1
Coordinates 34212..34442 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM361_RS24760 30843..31598 - 756 WP_000852146.1 replication initiation protein RepE -
AM361_RS24765 32320..33126 - 807 WP_000016970.1 site-specific integrase -
AM361_RS24770 33127..33432 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
AM361_RS24775 33434..33652 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
AM361_RS24790 34212..34442 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM361_RS24795 34439..34855 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
AM361_RS24800 34930..36495 + 1566 WP_001128474.1 AAA family ATPase -
AM361_RS24805 36480..37502 + 1023 WP_000361402.1 helicase UvrD -
AM361_RS24815 37756..38442 - 687 Protein_53 IS1 family transposase -
AM361_RS24820 38553..39410 - 858 WP_000968139.1 iron/manganese ABC transporter permease subunit SitD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / sul1 / qacE / cmlA1 / ant(3'')-Ia / ant(2'')-Ia / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id - 1..114267 114267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T77220 WP_001034044.1 NZ_CP021733:34439-34855 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T77220 NZ_CP021733:34439-34855 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT77220 WP_001261286.1 NZ_CP021733:34212-34442 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT77220 NZ_CP021733:34212-34442 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References