Detailed information of TA system TA077206

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4464370..4465205
• Replicon: chromosome
• Accession: NZ_CP021732
• Organism: Escherichia coli strain AR_0114

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: AM361_RS22785
• Protein ID: WP_042046912.1
• Coordinates: 4464370..4464747 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AM361_RS22790
• Protein ID: WP_073961352.1
• Coordinates: 4464837..4465205 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM361_RS22755	4459890..4460363	+	474	WP_001105407.1	DNA gyrase inhibitor SbmC	-
AM361_RS22760	4460561..4461619	+	1059	WP_001200895.1	FUSC family protein	-
AM361_RS22765	4461791..4462120	+	330	WP_000450409.1	DUF496 family protein	-
AM361_RS22770	4462221..4462403	-	183	WP_024198358.1	ethanolamine utilization protein	-
AM361_RS26635	4462857..4463246	-	390	WP_077875581.1	transposase	-
AM361_RS26435	4464044..4464157	-	114	WP_032140621.1	DUF957 domain-containing protein	-
AM361_RS22780	4464170..4464373	-	204	WP_000761705.1	hypothetical protein	-
AM361_RS22785	4464370..4464747	-	378	WP_042046912.1	TA system toxin CbtA family protein	Toxin
AM361_RS22790	4464837..4465205	-	369	WP_073961352.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM361_RS22800	4465368..4465589	-	222	WP_000692345.1	DUF987 domain-containing protein	-
AM361_RS22805	4465652..4466128	-	477	WP_001186775.1	RadC family protein	-
AM361_RS22810	4466144..4466617	-	474	WP_001350782.1	antirestriction protein	-
AM361_RS22820	4466959..4467777	-	819	WP_001234738.1	DUF945 domain-containing protein	-
AM361_RS22830	4467932..4468090	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4458613..4459833	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14020.96 Da
• Isoelectric Point: 7.8276

>T77206 WP_042046912.1 NZ_CP021732:c4464747-4464370 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T77206 NZ_CP021732:c4464747-4464370 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13444.17 Da
• Isoelectric Point: 6.2050

>AT77206 WP_073961352.1 NZ_CP021732:c4465205-4464837 [Escherichia coli]
VSDTLSGATHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT77206 NZ_CP021732:c4465205-4464837 [Escherichia coli]
GTGTCAGACACACTTTCCGGGGCAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA