Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3653528..3654111 Replicon chromosome
Accession NZ_CP021732
Organism Escherichia coli strain AR_0114

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag AM361_RS18935 Protein ID WP_000254738.1
Coordinates 3653776..3654111 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag AM361_RS18930 Protein ID WP_000581937.1
Coordinates 3653528..3653776 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM361_RS18920 3649867..3651168 + 1302 WP_001521172.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
AM361_RS18925 3651216..3653450 + 2235 WP_000226798.1 GTP diphosphokinase -
AM361_RS18930 3653528..3653776 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
AM361_RS18935 3653776..3654111 + 336 WP_000254738.1 endoribonuclease MazF Toxin
AM361_RS18940 3654183..3654974 + 792 WP_001071643.1 nucleoside triphosphate pyrophosphohydrolase -
AM361_RS18945 3655202..3656839 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
AM361_RS18950 3656926..3658224 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T77203 WP_000254738.1 NZ_CP021732:3653776-3654111 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T77203 NZ_CP021732:3653776-3654111 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT77203 WP_000581937.1 NZ_CP021732:3653528-3653776 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT77203 NZ_CP021732:3653528-3653776 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References