Detailed information of TA system TA077193

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1523006..1523700
• Replicon: chromosome
• Accession: NZ_CP021732
• Organism: Escherichia coli strain AR_0114

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: AM361_RS07935
• Protein ID: WP_001094398.1
• Coordinates: 1523006..1523374 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: AM361_RS07940
• Protein ID: WP_077249034.1
• Coordinates: 1523395..1523700 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM361_RS07915	1518179..1519135	+	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
AM361_RS07920	1519132..1521330	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
AM361_RS07925	1521340..1522296	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
AM361_RS07930	1522347..1522685	+	339	Protein_1445	LysR family transcriptional regulator	-
AM361_RS07935	1523006..1523374	-	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
AM361_RS07940	1523395..1523700	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM361_RS07945	1523804..1524448	-	645	WP_000086755.1	hypothetical protein	-
AM361_RS07950	1524467..1524688	-	222	WP_000692345.1	DUF987 domain-containing protein	-
AM361_RS07955	1524757..1525233	-	477	WP_001424026.1	RadC family protein	-
AM361_RS07960	1525249..1525722	-	474	WP_000855059.1	antirestriction protein	-
AM361_RS07970	1526064..1526885	-	822	WP_001234565.1	DUF945 domain-containing protein	-
AM361_RS26260	1526986..1527194	-	209	Protein_1453	DUF905 family protein	-
AM361_RS07980	1527295..1527750	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	1496517..1537349	40832

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T77193 WP_001094398.1 NZ_CP021732:c1523374-1523006 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T77193 NZ_CP021732:c1523374-1523006 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT77193 WP_077249034.1 NZ_CP021732:c1523700-1523395 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT77193 NZ_CP021732:c1523700-1523395 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4