Detailed information of TA system TA077185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1086174..1086952
• Replicon: chromosome
• Accession: NZ_CP021731
• Organism: Azoarcus sp. DN11

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: CDA09_RS04895
• Protein ID: WP_121427592.1
• Coordinates: 1086449..1086952 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: CDA09_RS04890
• Protein ID: WP_121427591.1
• Coordinates: 1086174..1086452 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDA09_RS04860	1082563..1082760	-	198	WP_121427585.1	hypothetical protein	-
CDA09_RS04865	1082909..1083565	-	657	WP_121427586.1	helix-turn-helix transcriptional regulator	-
CDA09_RS04870	1083674..1084111	-	438	WP_121427587.1	helix-turn-helix domain-containing protein	-
CDA09_RS04875	1084182..1084589	-	408	WP_121427588.1	type II toxin-antitoxin system VapC family toxin	-
CDA09_RS04880	1084589..1084843	-	255	WP_121427589.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
CDA09_RS04885	1085455..1086069	+	615	WP_121427590.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
CDA09_RS04890	1086174..1086452	+	279	WP_121427591.1	DUF1778 domain-containing protein	Antitoxin
CDA09_RS04895	1086449..1086952	+	504	WP_121427592.1	GNAT family N-acetyltransferase	Toxin
CDA09_RS04900	1087135..1087956	-	822	WP_121427593.1	IS21-like element helper ATPase IstB	-
CDA09_RS04905	1087949..1089442	-	1494	WP_121427594.1	IS21 family transposase	-
CDA09_RS04910	1089692..1090714	+	1023	WP_121427595.1	IS110 family transposase	-
CDA09_RS04915	1091027..1091203	+	177	Protein_967	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-88)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18394.16 Da
• Isoelectric Point: 7.3227

>T77185 WP_121427592.1 NZ_CP021731:1086449-1086952 [Azoarcus sp. DN11]
MSDGYSPVRKLLATDQVDAFDCGQAALNQFLQRYALVNQKANSAQTYVCCQSEVVVGFYSLAVGSVDPEAAPSRVIKGLA
RHPVPVMILARLAVDKAHHRQGLGQALLKDALLRTAQAADIAGIRCLLVHAKDDAARQWYESWEFEPSPTDPYHLFLMLK
DLKNLLS

Nucleotide

• Length: 504 bp

>T77185 NZ_CP021731:1086449-1086952 [Azoarcus sp. DN11]
TTGAGCGATGGCTACTCACCCGTTCGCAAGCTGCTTGCGACGGATCAGGTCGATGCTTTTGACTGCGGCCAGGCTGCGCT
GAACCAGTTCCTGCAGCGCTACGCGCTCGTCAACCAGAAGGCCAACAGCGCGCAGACCTACGTCTGCTGCCAGAGCGAGG
TGGTGGTCGGCTTCTACAGCCTCGCCGTCGGCAGCGTCGATCCGGAGGCGGCGCCGTCAAGAGTGATTAAGGGGCTCGCG
CGTCACCCGGTGCCGGTCATGATCCTCGCCCGGCTCGCGGTAGACAAGGCGCATCATCGCCAAGGCCTAGGCCAGGCCTT
ACTCAAGGATGCTCTGCTGCGCACCGCACAGGCCGCCGACATCGCCGGCATTCGTTGCCTGCTGGTCCATGCTAAGGACG
ATGCGGCACGGCAGTGGTACGAATCCTGGGAATTCGAGCCCAGCCCGACCGATCCGTACCATCTGTTCCTGATGCTCAAG
GATCTCAAGAATTTGCTGAGCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10228.71 Da
• Isoelectric Point: 10.4747

>AT77185 WP_121427591.1 NZ_CP021731:1086174-1086452 [Azoarcus sp. DN11]
MSAANLSKTERIDVRASTLIKQLLQDAARASHKNVSEFLLDAGVTAAAQTLADRRQFVLDETQWQAFQEALDRPVQSKPR
LKKLLREPGVLG

Nucleotide

• Length: 279 bp

>AT77185 NZ_CP021731:1086174-1086452 [Azoarcus sp. DN11]
ATGAGCGCTGCCAATCTCTCAAAAACCGAACGCATCGACGTTCGTGCAAGCACGCTGATCAAGCAGTTGTTGCAGGATGC
TGCACGTGCCAGCCACAAGAATGTCAGCGAGTTTCTGCTTGATGCAGGGGTGACGGCAGCCGCGCAGACGCTGGCGGATC
GTCGCCAGTTCGTGCTCGACGAGACGCAGTGGCAGGCGTTCCAGGAAGCGCTGGATCGGCCGGTGCAAAGCAAGCCGCGC
CTGAAGAAGTTGTTGCGTGAGCCCGGGGTGCTGGGTTGA