Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 47519..48120 Replicon plasmid pCombat11I9-3
Accession NZ_CP021729
Organism Escherichia coli strain Combat11I9

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag CDC27_RS28075 Protein ID WP_001216045.1
Coordinates 47519..47899 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag CDC27_RS28080 Protein ID WP_001190712.1
Coordinates 47899..48120 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDC27_RS28050 42959..44443 - 1485 WP_000124150.1 terminase -
CDC27_RS28055 44443..45636 - 1194 WP_106489616.1 terminase -
CDC27_RS28060 45723..46175 - 453 WP_001326849.1 late promoter-activating protein -
CDC27_RS28065 46264..47307 - 1044 WP_000648827.1 DUF968 domain-containing protein -
CDC27_RS28070 47335..47514 - 180 WP_001339207.1 hypothetical protein -
CDC27_RS28075 47519..47899 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CDC27_RS28080 47899..48120 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CDC27_RS28085 48193..48582 - 390 WP_000506730.1 DNA repair protein -
CDC27_RS28090 48757..49341 + 585 WP_044068914.1 hypothetical protein -
CDC27_RS28095 49342..49704 + 363 WP_044068912.1 hypothetical protein -
CDC27_RS28100 49716..49955 - 240 WP_023356286.1 DNA polymerase III subunit theta -
CDC27_RS28110 50270..51028 - 759 WP_032353390.1 hypothetical protein -
CDC27_RS28115 51025..51399 - 375 WP_032353387.1 hypothetical protein -
CDC27_RS28120 51406..51699 - 294 WP_000269001.1 hypothetical protein -
CDC27_RS28125 51878..52111 - 234 WP_000517421.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..114968 114968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T77184 WP_001216045.1 NZ_CP021729:c47899-47519 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T77184 NZ_CP021729:c47899-47519 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT77184 WP_001190712.1 NZ_CP021729:c48120-47899 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT77184 NZ_CP021729:c48120-47899 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References