Detailed information of TA system TA077183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 159971..160596
• Replicon: plasmid pCombat11I9-2
• Accession: NZ_CP021728
• Organism: Escherichia coli strain Combat11I9

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CDC27_RS27820
• Protein ID: WP_000911324.1
• Coordinates: 159971..160369 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: CDC27_RS27825
• Protein ID: WP_000450532.1
• Coordinates: 160369..160596 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDC27_RS27805	156279..156800	+	522	WP_000632670.1	conjugal transfer entry exclusion protein TraS	-
CDC27_RS27810	156825..157556	+	732	WP_000850422.1	complement resistance protein TraT	-
CDC27_RS27815	157809..159962	+	2154	WP_000009350.1	type IV conjugative transfer system coupling protein TraD	-
CDC27_RS27820	159971..160369	-	399	WP_000911324.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CDC27_RS27825	160369..160596	-	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / fosA3 / oqxB / oqxA / sul1 / sul2 / aph(3'')-Ib / aph(6)-Id / sitABCD	iroN / iroE / iroD / iroC / iroB	1..169487	169487

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14800.09 Da
• Isoelectric Point: 7.8604

>T77183 WP_000911324.1 NZ_CP021728:c160369-159971 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMEVIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELALQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T77183 NZ_CP021728:c160369-159971 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGGTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCTTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT77183 WP_000450532.1 NZ_CP021728:c160596-160369 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT77183 NZ_CP021728:c160596-160369 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5