Detailed information of TA system TA077178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 63207..63754
• Replicon: plasmid pCombat11I9-2
• Accession: NZ_CP021728
• Organism: Escherichia coli strain Combat11I9

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: CDC27_RS27150
• Protein ID: WP_042033558.1
• Coordinates: 63473..63754 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: CDC27_RS27145
• Protein ID: WP_000079941.1
• Coordinates: 63207..63476 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDC27_RS27135	59986..61260	-	1275	WP_000489607.1	HlyD family secretion protein	-
CDC27_RS27145	63207..63476	+	270	WP_000079941.1	hypothetical protein	Antitoxin
CDC27_RS27150	63473..63754	+	282	WP_042033558.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDC27_RS27155	63800..64648	+	849	WP_001058005.1	3'-5' exonuclease	-
CDC27_RS27160	64765..65247	+	483	WP_001311056.1	hypothetical protein	-
CDC27_RS27170	65549..65896	+	348	WP_000142437.1	hypothetical protein	-
CDC27_RS27175	66282..67313	-	1032	WP_001752311.1	IS630-like element ISEc33 family transposase	-
CDC27_RS27185	67610..68644	+	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / fosA3 / oqxB / oqxA / sul1 / sul2 / aph(3'')-Ib / aph(6)-Id / sitABCD	iroN / iroE / iroD / iroC / iroB	1..169487	169487
	-	inside	IScluster/Tn	-	-	61457..69866	8409

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10888.53 Da
• Isoelectric Point: 8.4615

>T77178 WP_042033558.1 NZ_CP021728:63473-63754 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYSASNLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRGLHTVQKWP

Nucleotide

• Length: 282 bp

>T77178 NZ_CP021728:63473-63754 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAACCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGGATTGCATACTGTACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT77178 WP_000079941.1 NZ_CP021728:63207-63476 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT77178 NZ_CP021728:63207-63476 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTACTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5