Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4654630..4655206 Replicon chromosome
Accession NZ_CP021726
Organism Escherichia coli strain Combat11I9

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag CDC27_RS23490 Protein ID WP_001295743.1
Coordinates 4654630..4654917 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A085P6S1
Locus tag CDC27_RS23495 Protein ID WP_001774708.1
Coordinates 4654904..4655206 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDC27_RS23475 4650215..4651594 - 1380 WP_033814748.1 restriction endonuclease subunit S -
CDC27_RS23480 4651584..4653203 - 1620 WP_033814747.1 type I restriction-modification system subunit M -
CDC27_RS23485 4653267..4654433 - 1167 WP_001672354.1 restriction endonuclease -
CDC27_RS23490 4654630..4654917 + 288 WP_001295743.1 BrnT family toxin Toxin
CDC27_RS23495 4654904..4655206 + 303 WP_001774708.1 BrnA antitoxin family protein Antitoxin
CDC27_RS23500 4655240..4656196 - 957 WP_001297640.1 GTPase -
CDC27_RS23505 4656207..4656410 - 204 WP_000467859.1 YbdD/YjiX family protein -
CDC27_RS23510 4656460..4658610 - 2151 WP_148119530.1 pyruvate/proton symporter BtsT -
CDC27_RS23520 4658903..4659445 - 543 WP_000934391.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T77175 WP_001295743.1 NZ_CP021726:4654630-4654917 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T77175 NZ_CP021726:4654630-4654917 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCT
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGTCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11502.00 Da        Isoelectric Point: 9.4646

>AT77175 WP_001774708.1 NZ_CP021726:4654904-4655206 [Escherichia coli]
MSMVKHKRGAPSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT77175 NZ_CP021726:4654904-4655206 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGGCACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A085P6S1

References