Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3025236..3025890 Replicon chromosome
Accession NZ_CP021726
Organism Escherichia coli strain Combat11I9

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag CDC27_RS15405 Protein ID WP_000244777.1
Coordinates 3025236..3025643 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CDC27_RS15410 Protein ID WP_000354046.1
Coordinates 3025624..3025890 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDC27_RS15385 3021193..3022926 - 1734 WP_000813236.1 single-stranded-DNA-specific exonuclease RecJ -
CDC27_RS15390 3022932..3023642 - 711 WP_000715218.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CDC27_RS15395 3023667..3024563 - 897 WP_000806630.1 site-specific tyrosine recombinase XerD -
CDC27_RS15400 3024675..3025196 + 522 WP_001055874.1 flavodoxin FldB -
CDC27_RS15405 3025236..3025643 - 408 WP_000244777.1 toxin CptA Toxin
CDC27_RS15410 3025624..3025890 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CDC27_RS15415 3026133..3027113 + 981 WP_000886100.1 tRNA-modifying protein YgfZ -
CDC27_RS15420 3027190..3027849 - 660 WP_000250270.1 hemolysin III family protein -
CDC27_RS15425 3028013..3028324 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
CDC27_RS15430 3028369..3029802 + 1434 WP_023308281.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T77169 WP_000244777.1 NZ_CP021726:c3025643-3025236 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T77169 NZ_CP021726:c3025643-3025236 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT77169 WP_000354046.1 NZ_CP021726:c3025890-3025624 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT77169 NZ_CP021726:c3025890-3025624 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTCAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References