Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1340345..1340983 Replicon chromosome
Accession NZ_CP021726
Organism Escherichia coli strain Combat11I9

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag CDC27_RS06860 Protein ID WP_000813794.1
Coordinates 1340345..1340521 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CDC27_RS06865 Protein ID WP_001270286.1
Coordinates 1340567..1340983 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDC27_RS06840 1335964..1337178 - 1215 WP_071591631.1 BenE family transporter YdcO -
CDC27_RS06845 1337231..1337767 + 537 WP_000429143.1 DNA-binding transcriptional regulator SutR -
CDC27_RS06850 1337840..1339801 + 1962 WP_021577429.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CDC27_RS06855 1339893..1340123 - 231 WP_000494244.1 YncJ family protein -
CDC27_RS06860 1340345..1340521 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CDC27_RS06865 1340567..1340983 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CDC27_RS06870 1341062..1342468 + 1407 WP_000760655.1 PLP-dependent aminotransferase family protein -
CDC27_RS06875 1342713..1343858 + 1146 WP_072686558.1 ABC transporter substrate-binding protein -
CDC27_RS06880 1343876..1344889 + 1014 WP_000220411.1 ABC transporter ATP-binding protein -
CDC27_RS06885 1344890..1345831 + 942 WP_021577433.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T77157 WP_000813794.1 NZ_CP021726:1340345-1340521 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T77157 NZ_CP021726:1340345-1340521 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT77157 WP_001270286.1 NZ_CP021726:1340567-1340983 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT77157 NZ_CP021726:1340567-1340983 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References