Detailed information of TA system TA077152

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 687071..687776
• Replicon: chromosome
• Accession: NZ_CP021726
• Organism: Escherichia coli strain Combat11I9

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A246P0D5
• Locus tag: CDC27_RS03505
• Protein ID: WP_000539522.1
• Coordinates: 687390..687776 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CDC27_RS03500
• Protein ID: WP_001280945.1
• Coordinates: 687071..687400 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDC27_RS03475	682787..684421	+	1635	Protein_623	CHASE9 sensor domain-containing protein	-
CDC27_RS03480	684437..685134	-	698	Protein_624	IS1 family transposase	-
CDC27_RS03490	685192..685665	+	474	Protein_625	EAL domain-containing protein	-
CDC27_RS03495	685673..687001	+	1329	WP_000086864.1	GGDEF domain-containing protein	-
CDC27_RS03500	687071..687400	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
CDC27_RS03505	687390..687776	-	387	WP_000539522.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDC27_RS03510	688002..689327	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
CDC27_RS03515	689540..689923	+	384	WP_000497137.1	biofilm formation regulator BssR	-
CDC27_RS03520	690034..691149	+	1116	WP_021577174.1	aldose sugar dehydrogenase YliI	-
CDC27_RS03525	691146..691772	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14292.51 Da
• Isoelectric Point: 10.2120

>T77152 WP_000539522.1 NZ_CP021726:c687776-687390 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIKDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T77152 NZ_CP021726:c687776-687390 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAAAGGATTTGATCGAAACCGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT77152 WP_001280945.1 NZ_CP021726:c687400-687071 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT77152 NZ_CP021726:c687400-687071 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A246P0D5