Detailed information of TA system TA077149

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4703279..4704114
• Replicon: chromosome
• Accession: NZ_CP021722
• Organism: Escherichia coli strain AR_0128

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7U9IW37
• Locus tag: AM375_RS26620
• Protein ID: WP_023356554.1
• Coordinates: 4703737..4704114 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9IW59
• Locus tag: AM375_RS26615
• Protein ID: WP_001360327.1
• Coordinates: 4703279..4703647 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM375_RS26575	4698943..4699623	+	681	WP_023356552.1	WYL domain-containing protein	-
AM375_RS26580	4699771..4700448	+	678	WP_001097302.1	hypothetical protein	-
AM375_RS26585	4700454..4700687	+	234	WP_001278293.1	DUF905 family protein	-
AM375_RS26590	4700777..4701595	+	819	WP_001175165.1	DUF945 domain-containing protein	-
AM375_RS26595	4701861..4702340	+	480	WP_000706978.1	antirestriction protein	-
AM375_RS26600	4702356..4702832	+	477	WP_001186774.1	RadC family protein	-
AM375_RS26605	4702895..4703116	+	222	WP_023356553.1	DUF987 domain-containing protein	-
AM375_RS26615	4703279..4703647	+	369	WP_001360327.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM375_RS26620	4703737..4704114	+	378	WP_023356554.1	TA system toxin CbtA family protein	Toxin
AM375_RS28290	4704111..4704260	+	150	Protein_4464	hypothetical protein	-
AM375_RS26625	4704339..4704533	+	195	WP_024181941.1	DUF957 domain-containing protein	-
AM375_RS26630	4704618..4705460	+	843	WP_022645115.1	DUF4942 domain-containing protein	-
AM375_RS26640	4706209..4707746	+	1538	Protein_4467	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4663199..4715559	52360

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14052.01 Da
• Isoelectric Point: 7.8045

>T77149 WP_023356554.1 NZ_CP021722:4703737-4704114 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFADERVIEQHIEAGIALCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T77149 NZ_CP021722:4703737-4704114 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTGCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.34 Da
• Isoelectric Point: 5.9598

>AT77149 WP_001360327.1 NZ_CP021722:4703279-4703647 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT77149 NZ_CP021722:4703279-4703647 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9IW37

Antitoxin

• AlphaFold DB: A0A7U9IW59