Detailed information of TA system TA077144

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2806043..2806681
• Replicon: chromosome
• Accession: NZ_CP021722
• Organism: Escherichia coli strain AR_0128

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: AM375_RS16735
• Protein ID: WP_000813794.1
• Coordinates: 2806505..2806681 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AM375_RS16730
• Protein ID: WP_001270286.1
• Coordinates: 2806043..2806459 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM375_RS16710	2801195..2802136	-	942	WP_001251315.1	ABC transporter permease	-
AM375_RS16715	2802137..2803150	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
AM375_RS16720	2803168..2804313	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
AM375_RS16725	2804558..2805964	-	1407	WP_000760592.1	PLP-dependent aminotransferase family protein	-
AM375_RS16730	2806043..2806459	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AM375_RS16735	2806505..2806681	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AM375_RS16740	2806903..2807133	+	231	WP_000494244.1	YncJ family protein	-
AM375_RS16745	2807225..2809186	-	1962	WP_001593323.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AM375_RS16750	2809259..2809795	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
AM375_RS16755	2809848..2811062	+	1215	WP_001348055.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2811102..2812250	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T77144 WP_000813794.1 NZ_CP021722:c2806681-2806505 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T77144 NZ_CP021722:c2806681-2806505 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT77144 WP_001270286.1 NZ_CP021722:c2806459-2806043 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT77144 NZ_CP021722:c2806459-2806043 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB