Detailed information of TA system TA077134

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2103981..2104816
• Replicon: chromosome
• Accession: NZ_CP021722
• Organism: Escherichia coli strain AR_0128

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1U9SHU4
• Locus tag: AM375_RS12985
• Protein ID: WP_000854752.1
• Coordinates: 2103981..2104358 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: AM375_RS12990
• Protein ID: WP_001278232.1
• Coordinates: 2104448..2104816 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM375_RS12955	2099501..2099974	+	474	WP_001105384.1	DNA gyrase inhibitor SbmC	-
AM375_RS12960	2100172..2101230	+	1059	WP_001200892.1	FUSC family protein	-
AM375_RS12965	2101402..2101731	+	330	WP_000450409.1	DUF496 family protein	-
AM375_RS12970	2101832..2102014	-	183	WP_024167529.1	ethanolamine utilization protein	-
AM375_RS28415	2102468..2102839	-	372	WP_001295631.1	IS110 family transposase	-
AM375_RS28070	2103655..2103768	-	114	WP_001161660.1	DUF957 domain-containing protein	-
AM375_RS12980	2103781..2103984	-	204	WP_000761705.1	hypothetical protein	-
AM375_RS12985	2103981..2104358	-	378	WP_000854752.1	TA system toxin CbtA family protein	Toxin
AM375_RS12990	2104448..2104816	-	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
AM375_RS13000	2104979..2105200	-	222	WP_000692329.1	DUF987 domain-containing protein	-
AM375_RS13005	2105263..2105739	-	477	WP_001347688.1	RadC family protein	-
AM375_RS13010	2105755..2106240	-	486	WP_000849582.1	antirestriction protein	-
AM375_RS13015	2106295..2107113	-	819	WP_001234620.1	DUF945 domain-containing protein	-
AM375_RS13025	2107213..2107446	-	234	WP_001119729.1	DUF905 family protein	-
AM375_RS13030	2107525..2107980	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14076.08 Da
• Isoelectric Point: 8.2905

>T77134 WP_000854752.1 NZ_CP021722:c2104358-2103981 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T77134 NZ_CP021722:c2104358-2103981 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCCCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT77134 WP_001278232.1 NZ_CP021722:c2104816-2104448 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT77134 NZ_CP021722:c2104816-2104448 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1U9SHU4

Antitoxin

• AlphaFold DB: E3PJ72