Detailed information of TA system TA077125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 115168..115769
• Replicon: plasmid tig00000793
• Accession: NZ_CP021720
• Organism: Escherichia coli strain AR_0128

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: AM375_RS01575
• Protein ID: WP_069067274.1
• Coordinates: 115389..115769 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: AM375_RS01570
• Protein ID: WP_001190712.1
• Coordinates: 115168..115389 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM375_RS01520	110378..111067	+	690	WP_069067277.1	hypothetical protein	-
AM375_RS01525	111054..111740	+	687	WP_080028522.1	ead/Ea22-like family protein	-
AM375_RS01530	111737..112450	+	714	WP_069067276.1	ead/Ea22-like family protein	-
AM375_RS28335	112450..112626	+	177	WP_001261535.1	hypothetical protein	-
AM375_RS01535	112623..112811	+	189	WP_000797282.1	hypothetical protein	-
AM375_RS01540	112813..113022	+	210	WP_000951710.1	hypothetical protein	-
AM375_RS01545	113019..113720	+	702	WP_074446721.1	hypothetical protein	-
AM375_RS01550	113704..113988	+	285	WP_001142396.1	hypothetical protein	-
AM375_RS01555	113973..114182	-	210	WP_001344848.1	hypothetical protein	-
AM375_RS01560	114356..114607	+	252	WP_032220968.1	DNA polymerase III subunit theta	-
AM375_RS01565	114706..115095	+	390	WP_069067275.1	DNA repair protein	-
AM375_RS01570	115168..115389	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AM375_RS01575	115389..115769	+	381	WP_069067274.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
AM375_RS01580	115853..116653	-	801	WP_000432092.1	hypothetical protein	-
AM375_RS01585	116660..117322	-	663	WP_069067273.1	DUF2829 domain-containing protein	-
AM375_RS01590	117337..117831	-	495	WP_000640903.1	dUTP diphosphatase	-
AM375_RS01595	117828..118304	-	477	WP_000861173.1	hypothetical protein	-
AM375_RS01600	118301..118609	-	309	WP_000349257.1	hypothetical protein	-
AM375_RS01605	118719..119747	-	1029	WP_080028523.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-1	mrkJ / mrkF / htpB	1..216378	216378

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-121)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13607.51 Da
• Isoelectric Point: 5.6406

>T77125 WP_069067274.1 NZ_CP021720:115389-115769 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGKAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Nucleotide

• Length: 381 bp

>T77125 NZ_CP021720:115389-115769 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAAGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGTATACAGGTATATGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCCGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGAATTATTTGGTTCCAATATTTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT77125 WP_001190712.1 NZ_CP021720:115168-115389 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT77125 NZ_CP021720:115168-115389 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTTGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6