Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 148005..148741 Replicon plasmid tig00000000
Accession NZ_CP021713
Organism Klebsiella pneumoniae strain AR_0129

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AM376_RS00875 Protein ID WP_003026803.1
Coordinates 148259..148741 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AM376_RS00870 Protein ID WP_003026799.1
Coordinates 148005..148271 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM376_RS00825 144067..144429 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
AM376_RS00830 144479..144829 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
AM376_RS00835 145187..145456 + 270 WP_004152102.1 hypothetical protein -
AM376_RS00840 145444..146019 + 576 WP_004152103.1 hypothetical protein -
AM376_RS00845 146050..146544 + 495 WP_004152104.1 DNA-binding protein -
AM376_RS00850 146588..146956 + 369 WP_004152105.1 hypothetical protein -
AM376_RS00855 146990..147193 + 204 WP_004152106.1 hemolysin expression modulator Hha -
AM376_RS00860 147242..147499 + 258 WP_004152107.1 hypothetical protein -
AM376_RS00865 147575..147829 + 255 WP_004152108.1 hypothetical protein -
AM376_RS00870 148005..148271 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AM376_RS00875 148259..148741 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AM376_RS00880 148953..150299 + 1347 WP_020314316.1 ISNCY family transposase -
AM376_RS00885 150800..152054 - 1255 Protein_153 IS3 family transposase -
AM376_RS00890 152142..153104 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / catA1 - 1..208224 208224


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T77104 WP_003026803.1 NZ_CP021713:148259-148741 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T77104 NZ_CP021713:148259-148741 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT77104 WP_003026799.1 NZ_CP021713:148005-148271 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT77104 NZ_CP021713:148005-148271 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References