Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 20556..21199 Replicon plasmid tig00000857
Accession NZ_CP021712
Organism Klebsiella pneumoniae strain AR_0143

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag AM390_RS30075 Protein ID WP_001044770.1
Coordinates 20556..20972 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag AM390_RS30080 Protein ID WP_001261282.1
Coordinates 20969..21199 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM390_RS30045 15724..16512 + 789 WP_001206315.1 ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1 -
AM390_RS30050 16572..17396 + 825 WP_000722315.1 oxacillin-hydrolyzing class D beta-lactamase OXA-9 -
AM390_RS30055 17596..17913 + 318 Protein_23 recombinase family protein -
AM390_RS30060 18096..18956 + 861 WP_000027057.1 class A broad-spectrum beta-lactamase TEM-1 -
AM390_RS30065 19041..19738 - 698 Protein_25 IS1 family transposase -
AM390_RS30070 19772..20482 - 711 WP_004152334.1 ATP-binding protein -
AM390_RS30075 20556..20972 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
AM390_RS30080 20969..21199 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM390_RS31340 21156..21617 + 462 WP_072202616.1 hypothetical protein -
AM390_RS30090 21761..22111 + 351 WP_000493378.1 hypothetical protein -
AM390_RS30095 22162..22905 + 744 WP_000129823.1 hypothetical protein -
AM390_RS30100 22902..23678 + 777 WP_000015958.1 site-specific integrase -
AM390_RS30105 23736..23993 - 258 WP_000764642.1 hypothetical protein -
AM390_RS31345 24122..24226 - 105 WP_032409716.1 hypothetical protein -
AM390_RS30110 24761..25627 + 867 WP_004118283.1 replication initiation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid ant(3'')-Ia / cmlA1 / qacE / sul1 / aac(6')-Ib / blaOXA-9 / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / blaCTX-M-15 / aac(3)-IId / blaSHV-187 / ARR-3 - 1..70457 70457
- inside Integron - - 2..70455 70453


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T77103 WP_001044770.1 NZ_CP021712:c20972-20556 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T77103 NZ_CP021712:c20972-20556 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT77103 WP_001261282.1 NZ_CP021712:c21199-20969 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT77103 NZ_CP021712:c21199-20969 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References