Detailed information of TA system TA077078

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 344886..345639
• Replicon: plasmid tig00000727
• Accession: NZ_CP021699
• Organism: Klebsiella pneumoniae strain AR_0158

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: R8WN26
• Locus tag: AM404_RS29300
• Protein ID: WP_016154457.1
• Coordinates: 344886..345263 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AM404_RS29305
• Protein ID: WP_162838704.1
• Coordinates: 345313..345639 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM404_RS29265	339908..340549	-	642	WP_009652489.1	TerD family protein	-
AM404_RS29270	340574..341212	-	639	WP_009652437.1	VWA domain-containing protein	-
AM404_RS29275	341532..342005	-	474	WP_009652494.1	ribbon-helix-helix protein, CopG family	-
AM404_RS29280	342008..343015	-	1008	Protein_353	hypothetical protein	-
AM404_RS29285	343239..344084	-	846	Protein_354	DUF4942 domain-containing protein	-
AM404_RS29290	344165..344368	-	204	WP_009652505.1	DUF957 domain-containing protein	-
AM404_RS29295	344398..344889	-	492	WP_009652438.1	hypothetical protein	-
AM404_RS29300	344886..345263	-	378	WP_016154457.1	TA system toxin CbtA family protein	Toxin
AM404_RS29305	345313..345639	-	327	WP_162838704.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM404_RS29310	345696..345917	-	222	WP_009652392.1	DUF987 domain-containing protein	-
AM404_RS29315	345931..346413	-	483	WP_016154455.1	DNA repair protein RadC	-
AM404_RS29320	346425..346643	-	219	WP_009652503.1	hypothetical protein	-
AM404_RS29325	346654..347115	-	462	WP_045890390.1	antirestriction protein	-
AM404_RS29330	347144..347398	-	255	WP_009652519.1	hypothetical protein	-
AM404_RS29335	347398..348216	-	819	WP_009652424.1	DUF945 domain-containing protein	-
AM404_RS29340	348320..348553	-	234	WP_009652474.1	DUF905 domain-containing protein	-
AM404_RS29345	348609..348830	-	222	WP_032950095.1	hypothetical protein	-
AM404_RS29350	348912..349424	-	513	WP_032950094.1	DUF4234 domain-containing protein	-
AM404_RS29355	349473..349943	-	471	WP_009652431.1	hypothetical protein	-
AM404_RS29360	349990..350301	-	312	WP_009652410.1	hypothetical protein	-
AM404_RS29365	350314..350580	-	267	WP_032950091.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaNDM-1 / tet(B) / blaOXA-1 / aac(6')-Ib-cr / qnrB1 / aac(3)-IId / blaSHV-12 / qacE / dfrA30	htpB	1..354705	354705

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14168.19 Da
• Isoelectric Point: 9.5224

>T77078 WP_016154457.1 NZ_CP021699:c345263-344886 [Klebsiella pneumoniae]
MQTQPVPPKREVSPRPSPVAIWQRLLSHLLDRHYGLTLNDTPFGKDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLTSIDILRARKATGLMTRHSYRAVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T77078 NZ_CP021699:c345263-344886 [Klebsiella pneumoniae]
ATGCAAACACAACCTGTTCCTCCGAAACGGGAGGTTTCGCCACGCCCGTCACCCGTGGCGATCTGGCAGCGCCTCCTGAG
TCACCTGCTGGACCGTCACTACGGTCTGACGCTCAACGACACGCCATTTGGCAAAGATGGTGTGATCCAAGAGCATATCG
ATGCCGGTATCTCACTGTGCGACGCGGTGAATTTCATCGTGGAGAAATACGACCTGGTACGCACTGATCGTCGCGGTTTT
AACGCGGAAACTCAGTCGCCGCTTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGCTGATGACCCGTCA
TAGCTACAGGGCCGTTACCGACATCACCACCGGCAAATACCGTGAGGTGCAACCATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12079.85 Da
• Isoelectric Point: 6.6283

>AT77078 WP_162838704.1 NZ_CP021699:c345639-345313 [Klebsiella pneumoniae]
IAAPWWGLKRDITPCFGARLVQEGNRLHYLNDRASITGTFSDADLRHLDQAFPLLLKQLELMLLSGELNPRHQHCVTLYA
KGLICEADSLGSHGYVYLAIYPTPATTE

Nucleotide

• Length: 327 bp

>AT77078 NZ_CP021699:c345639-345313 [Klebsiella pneumoniae]
ATCGCCGCCCCTTGGTGGGGGCTTAAACGCGATATCACCCCCTGTTTTGGCGCACGTCTGGTGCAGGAGGGCAACCGGCT
GCATTACCTGAACGACCGCGCCAGCATCACCGGCACCTTCAGCGACGCGGATTTACGCCATCTCGACCAGGCATTCCCGC
TACTGCTGAAGCAACTGGAGCTGATGCTGCTTTCCGGTGAGCTGAATCCCCGTCATCAGCACTGCGTCACGCTGTATGCA
AAAGGGCTGATATGCGAGGCCGACAGCCTCGGCTCGCACGGGTACGTCTATCTCGCAATTTACCCGACACCTGCAACAAC
AGAATAA

Structures

Toxin

• AlphaFold DB: R8WN26