Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 129813..130339 Replicon plasmid tig00000727
Accession NZ_CP021699
Organism Klebsiella pneumoniae strain AR_0158

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag AM404_RS28070 Protein ID WP_000323025.1
Coordinates 129813..130100 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag AM404_RS28075 Protein ID WP_000534858.1
Coordinates 130100..130339 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM404_RS28025 124860..125093 - 234 WP_004181905.1 hypothetical protein -
AM404_RS28030 125172..125519 - 348 WP_004181904.1 hypothetical protein -
AM404_RS28035 125675..126742 - 1068 WP_004181903.1 hypothetical protein -
AM404_RS28050 127431..127808 + 378 WP_004181901.1 hypothetical protein -
AM404_RS30335 128006..128980 - 975 WP_004181899.1 hypothetical protein -
AM404_RS28065 129583..129741 - 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
AM404_RS28070 129813..130100 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
AM404_RS28075 130100..130339 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
AM404_RS28085 130602..131525 - 924 WP_020277922.1 cation diffusion facilitator family transporter -
AM404_RS28090 131725..132297 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
AM404_RS28100 132773..134011 - 1239 WP_003030308.1 IS110 family transposase -
AM404_RS28105 134432..135067 - 636 Protein_138 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaNDM-1 / tet(B) / blaOXA-1 / aac(6')-Ib-cr / qnrB1 / aac(3)-IId / blaSHV-12 / qacE / dfrA30 htpB 1..354705 354705


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T77077 WP_000323025.1 NZ_CP021699:c130100-129813 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T77077 NZ_CP021699:c130100-129813 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT77077 WP_000534858.1 NZ_CP021699:c130339-130100 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT77077 NZ_CP021699:c130339-130100 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References