Detailed information of TA system TA077062

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2479039..2479822
• Replicon: chromosome
• Accession: NZ_CP021696
• Organism: Klebsiella pneumoniae strain AR_0158

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: AM404_RS12520
• Protein ID: WP_087728199.1
• Coordinates: 2479448..2479822 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AM404_RS12515
• Protein ID: WP_087728197.1
• Coordinates: 2479039..2479398 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM404_RS12470	2474665..2475342	+	678	WP_087728187.1	WYL domain-containing protein	-
AM404_RS12475	2475441..2475860	+	420	WP_087728488.1	hypothetical protein	-
AM404_RS12480	2475915..2476274	+	360	WP_040198488.1	hypothetical protein	-
AM404_RS12485	2476355..2476567	+	213	WP_010434180.1	hypothetical protein	-
AM404_RS12490	2476678..2477499	+	822	WP_087728188.1	DUF945 domain-containing protein	-
AM404_RS12495	2477579..2478058	+	480	WP_087728190.1	antirestriction protein	-
AM404_RS12500	2478069..2478302	+	234	WP_087728192.1	hypothetical protein	-
AM404_RS12505	2478299..2478781	+	483	WP_087728194.1	DNA repair protein RadC	-
AM404_RS12510	2478795..2479016	+	222	WP_087728196.1	DUF987 domain-containing protein	-
AM404_RS12515	2479039..2479398	+	360	WP_087728197.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM404_RS12520	2479448..2479822	+	375	WP_087728199.1	TA system toxin CbtA family protein	Toxin
AM404_RS12525	2480054..2481562	-	1509	WP_087728201.1	type I restriction-modification system subunit M	-
AM404_RS12530	2481564..2482367	-	804	WP_058709029.1	hypothetical protein	-
AM404_RS12535	2482364..2484088	-	1725	WP_087728203.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2470797..2484088	13291

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (9-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13911.91 Da
• Isoelectric Point: 10.3988

>T77062 WP_087728199.1 NZ_CP021696:2479448-2479822 [Klebsiella pneumoniae]
MQKQSLSPHRAASSRLSSVEIWRKLLKYLLEQHYGLTLNDTPFGNDSVIQKHIDAGISLCDAVNFIVEKYDLVRTDRHGF
SVKEQSPFIGSIDMLRARKATGLMTRNGYQTVNGIASGRFRGGK

Nucleotide

• Length: 375 bp

>T77062 NZ_CP021696:2479448-2479822 [Klebsiella pneumoniae]
ATGCAAAAACAATCCCTGTCCCCTCATAGGGCGGCCTCGTCACGTCTGTCATCAGTCGAAATCTGGCGAAAATTATTGAA
GTATCTGCTAGAGCAACACTACGGCCTTACCCTCAACGACACACCGTTTGGCAACGACAGTGTGATCCAGAAGCACATTG
ATGCGGGAATCTCACTATGTGACGCGGTGAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGACCGTCACGGCTTC
AGCGTGAAAGAGCAGTCTCCGTTTATCGGCAGCATTGATATGCTCCGCGCCAGGAAGGCTACCGGGCTGATGACACGCAA
TGGCTATCAAACCGTTAATGGTATCGCCAGTGGCAGATTCAGAGGAGGGAAATAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13237.32 Da
• Isoelectric Point: 7.7295

>AT77062 WP_087728197.1 NZ_CP021696:2479039-2479398 [Klebsiella pneumoniae]
MQKATSAANHNIAEPWWGLRRNVTPCFGARLVQEGNRLHYLADRASIAGTFNDADLRHLDQAFPVLMKQMELMLTSSELT
PRIQRCIMLHAKGLICEADTLGSCGYLFIVIYPTSATTA

Nucleotide

• Length: 360 bp

>AT77062 NZ_CP021696:2479039-2479398 [Klebsiella pneumoniae]
ATGCAGAAAGCAACCAGTGCAGCCAACCATAATATCGCGGAACCCTGGTGGGGCCTCAGACGCAACGTCACGCCGTGTTT
TGGTGCACGGCTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCTGACCGCGCCAGCATTGCCGGGACATTTAATGACG
CAGATTTACGCCATCTGGACCAGGCATTTCCGGTACTGATGAAGCAGATGGAGCTTATGTTGACCAGCAGCGAACTCACG
CCCCGCATTCAGCGCTGCATTATGCTCCACGCAAAAGGGCTGATATGTGAGGCCGATACGCTCGGCTCCTGTGGCTATTT
GTTCATCGTAATTTATCCGACATCTGCAACAACCGCATAA