Detailed information of TA system TA077061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1857582..1858172
• Replicon: chromosome
• Accession: NZ_CP021696
• Organism: Klebsiella pneumoniae strain AR_0158

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: AM404_RS09575
• Protein ID: WP_023341911.1
• Coordinates: 1857582..1857914 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: AM404_RS09580
• Protein ID: WP_000288812.1
• Coordinates: 1857915..1858172 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM404_RS09550	1853630..1853875	+	246	WP_061891617.1	signal transduction protein PmrD	-
AM404_RS09555	1854005..1855459	+	1455	WP_004148975.1	AMP nucleosidase	-
AM404_RS09565	1855825..1857261	-	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
AM404_RS09575	1857582..1857914	-	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AM404_RS09580	1857915..1858172	-	258	WP_000288812.1	antitoxin	Antitoxin
AM404_RS09590	1858507..1858713	-	207	WP_061891616.1	helix-turn-helix domain-containing protein	-
AM404_RS09595	1858710..1859170	-	461	Protein_1842	hypothetical protein	-
AM404_RS09600	1859453..1860010	-	558	WP_020805022.1	hypothetical protein	-
AM404_RS09605	1860089..1861837	-	1749	WP_087728091.1	hypothetical protein	-
AM404_RS09610	1862104..1862346	+	243	WP_004899316.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T77061 WP_023341911.1 NZ_CP021696:c1857914-1857582 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T77061 NZ_CP021696:c1857914-1857582 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT77061 WP_000288812.1 NZ_CP021696:c1858172-1857915 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT77061 NZ_CP021696:c1858172-1857915 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0