Detailed information of TA system TA077038

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4222794..4223488
• Replicon: chromosome
• Accession: NZ_CP021691
• Organism: Escherichia coli strain AR_0151

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: AM398_RS21590
• Protein ID: WP_001094398.1
• Coordinates: 4222794..4223162 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: AM398_RS21595
• Protein ID: WP_077249034.1
• Coordinates: 4223183..4223488 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM398_RS21570	4217967..4218923	+	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
AM398_RS21575	4218920..4221118	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
AM398_RS21580	4221128..4222084	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
AM398_RS21585	4222135..4222473	+	339	Protein_3907	LysR family transcriptional regulator	-
AM398_RS21590	4222794..4223162	-	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
AM398_RS21595	4223183..4223488	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM398_RS21600	4223592..4224236	-	645	WP_000086755.1	hypothetical protein	-
AM398_RS21605	4224255..4224476	-	222	WP_000692345.1	DUF987 domain-containing protein	-
AM398_RS21610	4224545..4225021	-	477	WP_001424026.1	RadC family protein	-
AM398_RS21615	4225037..4225510	-	474	WP_000855059.1	antirestriction protein	-
AM398_RS21625	4225852..4226673	-	822	WP_001234565.1	DUF945 domain-containing protein	-
AM398_RS25910	4226774..4226982	-	209	Protein_3915	DUF905 family protein	-
AM398_RS21635	4227083..4227538	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	4196304..4237137	40833

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T77038 WP_001094398.1 NZ_CP021691:c4223162-4222794 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T77038 NZ_CP021691:c4223162-4222794 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT77038 WP_077249034.1 NZ_CP021691:c4223488-4223183 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT77038 NZ_CP021691:c4223488-4223183 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4