Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1877070..1877590 Replicon chromosome
Accession NZ_CP021689
Organism Escherichia coli strain AR_0058

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag AM437_RS09620 Protein ID WP_001160964.1
Coordinates 1877306..1877590 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag AM437_RS09615 Protein ID WP_000125566.1
Coordinates 1877070..1877303 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM437_RS09590 1872143..1872430 + 288 WP_000203744.1 ferredoxin-like protein FixX -
AM437_RS09595 1872489..1873820 + 1332 WP_001183209.1 MFS transporter -
AM437_RS09600 1873928..1874458 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
AM437_RS09605 1874451..1876313 + 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
AM437_RS09610 1876505..1876984 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
AM437_RS09615 1877070..1877303 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AM437_RS09620 1877306..1877590 + 285 WP_001160964.1 CcdB family protein Toxin
AM437_RS09625 1877617..1878465 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
AM437_RS09630 1878472..1878849 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
AM437_RS09635 1878852..1879673 - 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
AM437_RS09640 1879670..1880659 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
AM437_RS09645 1880659..1881945 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T76990 WP_001160964.1 NZ_CP021689:1877306-1877590 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T76990 NZ_CP021689:1877306-1877590 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT76990 WP_000125566.1 NZ_CP021689:1877070-1877303 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT76990 NZ_CP021689:1877070-1877303 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References