Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 75536..76179 Replicon plasmid tig00001186
Accession NZ_CP021687
Organism Klebsiella pneumoniae strain AR_0146

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag AM393_RS28460 Protein ID WP_001044770.1
Coordinates 75763..76179 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag AM393_RS28455 Protein ID WP_001261282.1
Coordinates 75536..75766 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM393_RS28420 70849..71475 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
AM393_RS28425 71595..71774 + 180 WP_006788217.1 hypothetical protein -
AM393_RS28430 72238..73032 - 795 WP_015632375.1 site-specific integrase -
AM393_RS30035 73025..73186 - 162 WP_032495756.1 hypothetical protein -
AM393_RS28435 73230..74234 - 1005 WP_016162067.1 hypothetical protein -
AM393_RS28440 74299..74610 - 312 WP_024191724.1 hypothetical protein -
AM393_RS28445 74659..74973 - 315 WP_015632378.1 hypothetical protein -
AM393_RS30040 75118..75579 - 462 WP_165763053.1 hypothetical protein -
AM393_RS28455 75536..75766 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM393_RS28460 75763..76179 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
AM393_RS28465 76253..76963 + 711 WP_004152334.1 ATP-binding protein -
AM393_RS28470 76997..77694 + 698 Protein_87 IS1 family transposase -
AM393_RS28475 77711..77902 - 192 Protein_88 nucleotidyltransferase domain-containing protein -
AM393_RS28480 77972..78526 - 555 WP_063840280.1 AAC(6')-Ib family aminoglycoside 6'-N-acetyltransferase -
AM393_RS28485 78760..79317 - 558 WP_001217881.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-1 / aac(6')-Ib - 1..96814 96814


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T76980 WP_001044770.1 NZ_CP021687:75763-76179 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T76980 NZ_CP021687:75763-76179 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT76980 WP_001261282.1 NZ_CP021687:75536-75766 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT76980 NZ_CP021687:75536-75766 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References