Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 64549..65072 Replicon plasmid tig00001186
Accession NZ_CP021687
Organism Klebsiella pneumoniae strain AR_0146

Toxin (Protein)


Gene name ccdB Uniprot ID V5RNQ6
Locus tag AM393_RS28385 Protein ID WP_015632464.1
Coordinates 64549..64854 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V5RQR8
Locus tag AM393_RS28390 Protein ID WP_015632465.1
Coordinates 64854..65072 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM393_RS28340 59579..60007 - 429 WP_015632456.1 plasmid partitioning/stability family protein -
AM393_RS28345 60000..60980 - 981 WP_015632457.1 partitioning protein -
AM393_RS28350 61341..61523 + 183 WP_015632458.1 type II toxin-antitoxin system HicA family toxin -
AM393_RS28355 61549..61992 + 444 WP_015632459.1 type II toxin-antitoxin system HicB family antitoxin -
AM393_RS28360 62198..62320 - 123 Protein_64 transcriptional regulator -
AM393_RS28365 62334..62561 - 228 WP_016162072.1 hypothetical protein -
AM393_RS28370 62991..63248 + 258 WP_016162071.1 helix-turn-helix domain-containing protein -
AM393_RS28375 63452..64018 - 567 WP_015632462.1 hypothetical protein -
AM393_RS28380 64060..64404 - 345 WP_015632463.1 hypothetical protein -
AM393_RS28385 64549..64854 - 306 WP_015632464.1 type II toxin-antitoxin system toxin CcdB Toxin
AM393_RS28390 64854..65072 - 219 WP_015632465.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AM393_RS28395 65258..66283 + 1026 WP_015632466.1 IS110 family transposase -
AM393_RS28400 66578..67063 + 486 Protein_72 IS5 family transposase -
AM393_RS28405 67227..67658 + 432 WP_015632467.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
AM393_RS28410 67658..68929 + 1272 WP_016162068.1 Y-family DNA polymerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-1 / aac(6')-Ib - 1..96814 96814
- flank IS/Tn - - 65258..66283 1025


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11361.90 Da        Isoelectric Point: 5.5252

>T76979 WP_015632464.1 NZ_CP021687:c64854-64549 [Klebsiella pneumoniae]
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI

Download         Length: 306 bp

>T76979 NZ_CP021687:c64854-64549 [Klebsiella pneumoniae]
ATGCAGTTCACCGTCTACGAATACACACGCGAGAGCCATTATCGTCTTTTTGTGGATGTGCAGAGCGACATTGTCGAGAC
GCCTGGGCGACGCATAGTGATCCCGTTGCTGGAATCGCACCACTTATCAGAAAAGGTTAATCAGCAGCTTTTCCCGGTGG
TTAGCGTCAATGGGAAAGAATACCGTGCCGTGACTTCCGAATTATCCGGAGTCTCTGTCAGTGTCATCGGCGACGCTGTG
GCCGATCTCAGCCATGAGGCAAATGCCATTAAAAATGCCCTGAACCTGATGTTCTGGGGCATTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8194.13 Da        Isoelectric Point: 6.2224

>AT76979 WP_015632465.1 NZ_CP021687:c65072-64854 [Klebsiella pneumoniae]
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW

Download         Length: 219 bp

>AT76979 NZ_CP021687:c65072-64854 [Klebsiella pneumoniae]
ATGAAACACAGAGCCAGCGTCACAGTAGACAAGGAACATTATCTGGAATTACTCGCGGCAGGCGTGAATATTTCGGGACT
GGTGAATGAGGCTATCGGACGGGAAGCCCGGCGCATCCAGGCTGAACGCTGGAAAGCAGAGAACCGGGAAGGGATGGAAG
AGGTAGCGAATTTCATCGCGCAGCACGGCTCTTTTGCCGACGAAAACCGGAGCTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0ND19

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0L1T7

References