Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 104013..104749 Replicon plasmid tig00001160
Accession NZ_CP021686
Organism Klebsiella pneumoniae strain AR_0146

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AM393_RS27635 Protein ID WP_003026803.1
Coordinates 104267..104749 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AM393_RS27630 Protein ID WP_003026799.1
Coordinates 104013..104279 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM393_RS27590 99104..99451 + 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -
AM393_RS27595 99500..101038 + 1539 WP_004201219.1 IS66-like element ISKpn24 family transposase -
AM393_RS27610 101937..102245 - 309 WP_017896554.1 hypothetical protein -
AM393_RS27620 102739..103287 + 549 WP_001567366.1 thioredoxin fold domain-containing protein -
AM393_RS27625 103334..103768 + 435 WP_001567367.1 cell envelope integrity protein TolA -
AM393_RS27630 104013..104279 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AM393_RS27635 104267..104749 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AM393_RS27640 104950..106353 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
AM393_RS27645 106382..107014 - 633 WP_001567369.1 hypothetical protein -
AM393_RS27650 107228..107434 + 207 Protein_116 helix-turn-helix domain-containing protein -
AM393_RS27655 107632..109395 + 1764 WP_004200842.1 DUF262 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / mph(A) - 1..183376 183376
- inside IScluster/Tn - - 98703..106353 7650


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T76978 WP_003026803.1 NZ_CP021686:104267-104749 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T76978 NZ_CP021686:104267-104749 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT76978 WP_003026799.1 NZ_CP021686:104013-104279 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT76978 NZ_CP021686:104013-104279 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATTCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References