Detailed information of TA system TA076950

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3317365..3318059
• Replicon: chromosome
• Accession: NZ_CP021683
• Organism: Escherichia coli strain AR_0162

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: AM408_RS18105
• Protein ID: WP_001094398.1
• Coordinates: 3317365..3317733 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: AM408_RS18110
• Protein ID: WP_077249034.1
• Coordinates: 3317754..3318059 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM408_RS18085	3312538..3313494	+	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
AM408_RS18090	3313491..3315689	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
AM408_RS18095	3315699..3316655	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
AM408_RS18100	3316706..3317044	+	339	Protein_3119	LysR family transcriptional regulator	-
AM408_RS18105	3317365..3317733	-	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
AM408_RS18110	3317754..3318059	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM408_RS18115	3318163..3318807	-	645	WP_000086755.1	hypothetical protein	-
AM408_RS18120	3318826..3319047	-	222	WP_000692345.1	DUF987 domain-containing protein	-
AM408_RS18125	3319116..3319592	-	477	WP_001424026.1	RadC family protein	-
AM408_RS18130	3319608..3320081	-	474	WP_000855059.1	antirestriction protein	-
AM408_RS18140	3320423..3321244	-	822	WP_001234565.1	DUF945 domain-containing protein	-
AM408_RS26580	3321345..3321553	-	209	Protein_3127	DUF905 family protein	-
AM408_RS18150	3321654..3322109	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3290875..3331708	40833

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T76950 WP_001094398.1 NZ_CP021683:c3317733-3317365 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T76950 NZ_CP021683:c3317733-3317365 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT76950 WP_077249034.1 NZ_CP021683:c3318059-3317754 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT76950 NZ_CP021683:c3318059-3317754 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4