Detailed information of TA system TA076948

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2099559..2100197
• Replicon: chromosome
• Accession: NZ_CP021683
• Organism: Escherichia coli strain AR_0162

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: AM408_RS11780
• Protein ID: WP_001447010.1
• Coordinates: 2100021..2100197 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AM408_RS11775
• Protein ID: WP_001270286.1
• Coordinates: 2099559..2099975 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM408_RS11755	2094711..2095652	-	942	WP_001251304.1	ABC transporter permease	-
AM408_RS11760	2095653..2096666	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
AM408_RS11765	2096684..2097829	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
AM408_RS11770	2098074..2099480	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
AM408_RS11775	2099559..2099975	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AM408_RS11780	2100021..2100197	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AM408_RS11785	2100419..2100649	+	231	WP_000494244.1	YncJ family protein	-
AM408_RS11790	2100741..2102702	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AM408_RS11795	2102775..2103311	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
AM408_RS11800	2103364..2104578	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T76948 WP_001447010.1 NZ_CP021683:c2100197-2100021 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T76948 NZ_CP021683:c2100197-2100021 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT76948 WP_001270286.1 NZ_CP021683:c2099975-2099559 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT76948 NZ_CP021683:c2099975-2099559 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8